Fast detection of alpha-fetoprotein-L3 using lens culinaris agglutinin immobilized gold nanoparticles.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Sun Y; Qin L; Liu D; Liu C; Sun Y; Duan Y
  • Source:
    Journal of nanoscience and nanotechnology [J Nanosci Nanotechnol] 2014 Jun; Vol. 14 (6), pp. 4078-81.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Scientific Publishers Country of Publication: United States NLM ID: 101088195 Publication Model: Print Cited Medium: Print ISSN: 1533-4880 (Print) Linking ISSN: 15334880 NLM ISO Abbreviation: J Nanosci Nanotechnol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Stevenson Ranch, CA : American Scientific Publishers, 2001-2021
    • Subject Terms:
      Gold/*chemistry ; Metal Nanoparticles/*chemistry ; Microscopy, Electron/*methods ; Nanoconjugates/*chemistry ; Plant Lectins/*chemistry ; Spectrophotometry, Ultraviolet/*methods ; alpha-Fetoproteins/*analysis; Adsorption ; Biomarkers, Tumor/analysis ; Gold/analysis ; Metal Nanoparticles/analysis ; Protein Binding ; alpha-Fetoproteins/chemistry
    • Abstract:
      Colloidal gold is extensively used for molecular sensing because that the surface plasmon resonance (SPR) bands are affected by changes in the dielectric properties in the close vicinity of these structures due to the binding of ligands to the corresponding receptor molecules immobilized onto the nanostructures through chemi- or physisorption. We describe a simple method for the detection of Alpha-Fetoprotein-L3 which is a new generation of tumor marker for hepatocellular carcinoma (HCC) based on the aggregation of Lens culinaris agglutinin (LCA) Immobilized Gold Nanoparticles. The LCA conjugated GNPs obtained were 15-20 nm in diameter. The visible color change of the gold nanoparticles from purple to blue on interaction with 100 ng/mL of AFP-L3 is the principle applied here for the sensing of AFP-L3 level. UV/Vis spectroscopy also allows assay monitoring by quantifying the red shift of the plasmon resonance wavelength. With this method, the protein AFP-L3 can be rapidly detected as demanded for clinical diagnosis.
    • Accession Number:
      0 (Biomarkers, Tumor)
      0 (Nanoconjugates)
      0 (Plant Lectins)
      0 (alpha-Fetoproteins)
      0 (lentil lectin)
      7440-57-5 (Gold)
    • Publication Date:
      Date Created: 20140418 Date Completed: 20140508 Latest Revision: 20190715
    • Publication Date:
      20231215
    • Accession Number:
      10.1166/jnn.2014.8652
    • Accession Number:
      24738354