Effect of desiccation level and storage temperature on green spore viability of Osmunda japonica.

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  • Author(s): Li Y;Li Y; Shi L; Shi L
  • Source:
    Cryobiology [Cryobiology] 2014 Jun; Vol. 68 (3), pp. 446-50. Date of Electronic Publication: 2014 Mar 19.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE
    • Publication Information:
      Publication: <2000- > : Amsterdam : Elsevier
      Original Publication: San Diego, CA : Academic Press
    • Subject Terms:
      Desiccation*/methods ; Germination* ; Preservation, Biological*/methods; Ferns/*growth & development; Ferns/chemistry ; Spores/chemistry ; Spores/growth & development ; Temperature ; Water/chemistry
    • Abstract:
      In order to effectively preserve green spores, which have relatively higher water content and lose viability more quickly than non-green spores, we studied the effect of desiccation level and storage temperature on Osmunda japonica spores. The water content of fresh spores was 11.20%. After 12h desiccation by silica gel, the water content decreased to 6% but spore viability did not change significantly. As the desiccation continued, the decrease in water content slowed, but spore viability dropped. For almost all storage periods, the effects of storage temperature, desiccation level, and temperature×desiccation level were significantly different. After seven days of storage, spores at any desiccation level stored at 4°C obtained high germination rates. After more than seven days storage, liquid nitrogen (LN) storage obtained the best results. Storage at -18°C led to the lowest germination rates. Spores stored at room temperature and -18°C all died within three months. For storage at 4°C and in LN, spores desiccated 12 and 36 h obtained better results. Spores without desiccation had the highest germination rates after being stored at room temperature, but suffered the greatest loss after storage at -18°C. These results suggest that LN storage is the best method of long-term storage of O. japonica spores. The critical water content of O. japonica spores is about 6% and reduction of the water content to this level improves outcome after LN storage greatly. The reason for various responses of O. japonica spores to desiccation and storage temperatures are discussed.
      (Copyright © 2014 Elsevier Inc. All rights reserved.)
    • Contributed Indexing:
      Keywords: Desiccation; Green spore; Liquid nitrogen (LN) storage; Osmunda japonica; Spore viability
    • Accession Number:
      059QF0KO0R (Water)
    • Publication Date:
      Date Created: 20140325 Date Completed: 20150105 Latest Revision: 20140527
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.cryobiol.2014.03.002
    • Accession Number:
      24657199