Edwardsiella tarda Hfq: impact on host infection and global protein expression.

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  • Author(s): Hu YH; Li YX; Sun L; Sun L
  • Source:
    Veterinary research [Vet Res] 2014 Feb 25; Vol. 45, pp. 23. Date of Electronic Publication: 2014 Feb 25.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 9309551 Publication Model: Electronic Cited Medium: Internet ISSN: 1297-9716 (Electronic) Linking ISSN: 09284249 NLM ISO Abbreviation: Vet Res Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : London : BioMed Central
      Original Publication: Paris : Editions Scientifiques Elsevier ; INRA, c1993-
    • Subject Terms:
      Flounder* ; Gene Expression Regulation, Bacterial*; Edwardsiella tarda/*pathogenicity ; Edwardsiella tarda/*physiology ; Enterobacteriaceae Infections/*veterinary ; Fish Diseases/*microbiology ; Host Factor 1 Protein/*genetics ; Viral Vaccines/*pharmacology; Animals ; Edwardsiella tarda/genetics ; Edwardsiella tarda/immunology ; Enterobacteriaceae Infections/genetics ; Enterobacteriaceae Infections/microbiology ; Enterobacteriaceae Infections/prevention & control ; Fish Diseases/genetics ; Fish Diseases/prevention & control ; Host Factor 1 Protein/metabolism ; Real-Time Polymerase Chain Reaction/veterinary ; Reverse Transcriptase Polymerase Chain Reaction/veterinary ; Virulence
    • Abstract:
      Hfq is an RNA-binding protein that plays an important role in many cellular processes. In this study, we examined the biological effect of the Hfq of Edwardsiella tarda, a severe fish pathogen with a broad host range that includes humans. To facilitate the study, a markerless hfq in-frame deletion wild type, TXhfq, was constructed. Compared to the wild type TX01, TXhfq exhibited (i) retarded planktonic and biofilm growth, (ii) decreased resistance against oxidative stress, (iii) attenuated overall virulence and tissue dissemination and colonization capacity, (iv) impaired ability to replicate in host macrophages and to block host immune response. Introduction of a trans-expressed hfq gene into TXhfq restored the lost virulence of TXhfq. To identify potential Hfq targets, comparative global proteomic analysis was conducted, which revealed that 20 proteins belonging to different functional categories were differentially expressed in TXhfq and TX01. Quantitative real time RT-PCR analysis showed that the mRNA levels of two thirds of the genes of the identified proteins were consistent with the proteomic results. Since TXhfq is dramatically attenuated in virulence, we further examined its potential as a naturally delivered vaccine administered via the immersion route in a flounder model. The results showed that TXhfq induced effective protection against lethal E. tarda challenge. Taken together, our study indicated that Hfq is required for the normal operation of E. tarda in multiple aspects, and that Hfq probably exerts a regulatory effect on a wide range of target genes at both transcription and post-transcription levels.
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    • Accession Number:
      0 (Host Factor 1 Protein)
      0 (Viral Vaccines)
    • Publication Date:
      Date Created: 20140227 Date Completed: 20140915 Latest Revision: 20211021
    • Publication Date:
      20231215
    • Accession Number:
      PMC4015145
    • Accession Number:
      10.1186/1297-9716-45-23
    • Accession Number:
      24568370