Genomic sequences of a low passage herpes simplex virus 2 clinical isolate and its plaque-purified derivative strain.

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  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 0110674 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0341 (Electronic) Linking ISSN: 00426822 NLM ISO Abbreviation: Virology Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Herpes Genitalis/*virology ; Herpesvirus 2, Human/*genetics; Amino Acid Sequence ; Base Sequence ; Genome, Viral ; Genomics ; Herpesvirus 2, Human/growth & development ; Herpesvirus 2, Human/isolation & purification ; Humans ; Male ; Molecular Sequence Data ; Open Reading Frames ; Serial Passage ; Viral Plaque Assay
    • Abstract:
      Herpes simplex virus 2 is an important human pathogen as the causative agent of genital herpes, neonatal herpes, and increased risk of HIV acquisition and transmission. Nevertheless, the only genomic sequence that has been completed is the attenuated HSV-2 HG52 laboratory strain. In this study we defined the genomic sequence of the HSV-2 SD90e low passage clinical isolate and a plaque-purified derivative, SD90-3P. We found minimal sequence differences between SD90e and SD90-3P. However, in comparisons with the HSV-2 HG52 reference genome sequence, the SD90e genome ORFs contained numerous point mutations, 13 insertions/deletions (indels), and 9 short compensatory frameshifts. The indels were true sequence differences, but the compensatory frameshifts were likely sequence errors in the original HG52 sequence. Because HG52 virus is less virulent than other HSV-2 strains and may not be representative of wildtype HSV-2 strains, we propose that the HSV-2 SD90e genome serve as the new HSV-2 reference genome.
      (Copyright © 2013 Elsevier Inc. All rights reserved.)
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    • Grant Information:
      HHSN272200900018C United States AI NIAID NIH HHS; R01 AI057552 United States AI NIAID NIH HHS; R56 AI057552 United States AI NIAID NIH HHS; AI057552 United States AI NIAID NIH HHS
    • Contributed Indexing:
      Keywords: DNA virus genome; Genital herpes; Herpes simplex virus 2; Sexually transmitted disease
    • Publication Date:
      Date Created: 20140208 Date Completed: 20140502 Latest Revision: 20211021
    • Publication Date:
      20240829
    • Accession Number:
      PMC3955984
    • Accession Number:
      10.1016/j.virol.2013.12.014
    • Accession Number:
      24503076