Evaluation of micro-colorimetric lipid determination method with samples prepared using sonication and accelerated solvent extraction methods.

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  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE
    • Publication Information:
      Publication: Amsterdam : Elsevier
      Original Publication: Oxford : Pergamon Press
    • Subject Terms:
      Sonication*; Colorimetry/*methods ; Lipids/*analysis ; Solvents/*chemistry; Glycine max/chemistry
    • Abstract:
      Two common laboratory extraction techniques were evaluated for routine use with the micro-colorimetric lipid determination method developed by Van Handel (1985) [2] and recently validated for small samples by Inouye and Lotufo (2006) [1]. With the accelerated solvent extraction method using chloroform:methanol solvent and the colorimetric lipid determination method, 28 of 30 samples had significant proportional bias (α=1%, determined using standard additions) and 1 of 30 samples had significant constant bias (α=1%, determined using Youden Blank measurements). With sonic extraction, 0 of 6 samples had significant proportional bias (α=1%) and 1 of 6 samples had significant constant bias (α=1%). These demonstrate that the accelerated solvent extraction method with chloroform:methanol solvent system creates an interference with the colorimetric assay method, and without accounting for the bias in the analysis, inaccurate measurements would be obtained.
      (Published by Elsevier B.V.)
    • Contributed Indexing:
      Keywords: Accelerated solvent extraction; Lipid measurement; Micro-colorimetric
    • Accession Number:
      0 (Lipids)
      0 (Solvents)
    • Publication Date:
      Date Created: 20140110 Date Completed: 20140902 Latest Revision: 20231213
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.talanta.2013.09.051
    • Accession Number:
      24401464