Two ancestral APETALA3 homologs from the basal angiosperm Magnolia wufengensis (Magnoliaceae) can affect flower development of Arabidopsis.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
    • Publication Information:
      Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
    • Subject Terms:
      Arabidopsis/*genetics ; Arabidopsis/*growth & development ; Arabidopsis Proteins/*genetics ; Flowers/*growth & development ; MADS Domain Proteins/*genetics ; Magnolia/*genetics ; Plant Proteins/*genetics; Amino Acid Sequence ; Cloning, Molecular ; Flowers/genetics ; Gene Expression Regulation, Plant ; Molecular Sequence Data ; Mutation ; Phylogeny ; Plant Proteins/metabolism ; Plants, Genetically Modified ; Sequence Homology, Amino Acid
    • Abstract:
      APETALA3 (AP3) homologs are involved in specifying petal and stamen identities in core eudicot model organisms. In order to investigate the functional conservation of AP3 homologs between core eudicots and basal angiosperm, we isolated and identified two AP3 homologs from Magnolia wufengensis, a woody basal angiosperm belonging to the family Magnoliaceae. Sequence and phylogenetic analyses revealed that both genes are clade members of the paleoAP3 lineage. Moreover, a highly conserved motif of paleoAP3 is found in the C-terminal regions of MAwuAP3_1/2 proteins, but the PI-derived motif, usually present in AP3/DEF-like lineage members, is missing. Semi-quantitative and real time PCR analyses showed that the expression of MAwuAP3_1/2 was restricted to tepals and stamens. However, the MAwuAP3_1 expression was maintained at a high level during the rapid increased in size of tepals and stamens, while MAwuAP3_2 mRNA was only detected at the early stage of tepal and stamen development. Furthermore, the expression of MAwuAP3_1/2 in transgenic Arabidopsis causes phenotypic changes which partly resemble those caused by ectopic expressions of the endogenous AP3 gene. Moreover, the 35S::MAwuAP3_1/2 transgenic Arabidopsis can be used partially to rescue the loss-of-function ap3 mutant (ap3-3) of Arabidopsis. These findings call for a more comprehensive understanding of the B-functional evolution from basal angiosperm to core eudicot clades.
      (Copyright © 2013 Elsevier B.V. All rights reserved.)
    • Contributed Indexing:
      Keywords: AP3; AP3/DEF; APETALA3; APETALA3/DEFICIENS; B-class MADS box gene; BLAST; Basic Local Alignment Search Tool; DNA complementary to RNA; Dnase; Flower development; Magnolia wufengensis; ORF; PCR; PI; PISTILLATA; Polymerase chain reaction; QPCR; RACE; RNase; RT-PCR; UTR; base pair(s); bp; cDNA; dCAPS; deoxyribonuclease; derived cleaved amplified polymorphic sequence; open reading frame; quantitative polymerase chain reaction; rapid amplification of cDNA ends; reverse transcription polymerase chain reaction; ribonuclease; untranslated region(s)
    • Molecular Sequence:
      GENBANK JN315681
    • Accession Number:
      0 (APETALA 3 protein, Arabidopsis)
      0 (Arabidopsis Proteins)
      0 (MADS Domain Proteins)
      0 (Plant Proteins)
    • Publication Date:
      Date Created: 20131217 Date Completed: 20140404 Latest Revision: 20140127
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.gene.2013.11.076
    • Accession Number:
      24334124