Design of a fluidic circuit-based microcytometer for circulating tumor cell detection and enumeration.

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  • Author(s): Guo J; Lei W; Ma X; Xue P; Chen Y; Kang Y
  • Source:
    IEEE transactions on biomedical circuits and systems [IEEE Trans Biomed Circuits Syst] 2014 Feb; Vol. 8 (1), pp. 35-41. Date of Electronic Publication: 2013 Sep 13.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: IEEE Country of Publication: United States NLM ID: 101312520 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1940-9990 (Electronic) Linking ISSN: 19324545 NLM ISO Abbreviation: IEEE Trans Biomed Circuits Syst Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, NY : IEEE, c2007-
    • Subject Terms:
      Neoplastic Cells, Circulating*; Cell Separation/*instrumentation ; Microfluidic Analytical Techniques/*instrumentation; Computer Simulation ; Dimethylpolysiloxanes ; Equipment Design ; Erythrocytes ; Humans ; MCF-7 Cells ; Nylons
    • Abstract:
      Portable devices have been introduced to provide companion diagnostics in many applications such as personalized healthcare monitoring since several decades ago. Recently the polydimethylsiloxane (PDMS)-based microfluidic chip enables a cost effective platform for point of care diagnostics. In this paper, we present a systematic theoretical and experimental study of a novel fluidic circuit-based microcytometer. The working principle of this device is based on the characterization of the bandwidth and amplitude of the bias-voltage pulses induced by the microparticle's physical blockage of the sensing aperture. In the simulation, the amplitude and bandwidth of the bias voltage change is simply related to the microparticle translocation time and resistance change in the sensing aperture. In the modeling part, we simulate the two parameters (peak and translocation time) by considering 7 μm and 16 μm, which is used to approximately characterize the Red Blood Cells (RBCs) and Circulating Tumor Cells (CTCs). In the experimental setup, microparticles of different sizes are used to demonstrate the chip performance. Furthermore, RBCs and CTCs are detected and enumerated by the proposed chip. The microcytometry chip is presented and is expected toward the point of care clinical diagnostics.
    • Accession Number:
      0 (Dimethylpolysiloxanes)
      0 (Nylons)
      0 (poly(dimethylsiloxane)-polyamide copolymer)
    • Publication Date:
      Date Created: 20130920 Date Completed: 20150129 Latest Revision: 20140331
    • Publication Date:
      20221213
    • Accession Number:
      10.1109/TBCAS.2013.2275091
    • Accession Number:
      24048075