Rapid and tunable control of protein stability in Caenorhabditis elegans using a small molecule.

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  • Author(s): Cho U;Cho U; Zimmerman SM; Chen LC; Owen E; Kim JV; Kim SK; Wandless TJ
  • Source:
    PloS one [PLoS One] 2013 Aug 22; Vol. 8 (8), pp. e72393. Date of Electronic Publication: 2013 Aug 22 (Print Publication: 2013).
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Protein Stability*; Caenorhabditis elegans/*metabolism ; Caenorhabditis elegans Proteins/*metabolism; Animals ; Mice ; NIH 3T3 Cells ; Tetrahydrofolate Dehydrogenase/genetics ; Tetrahydrofolate Dehydrogenase/metabolism
    • Abstract:
      Destabilizing domains are conditionally unstable protein domains that can be fused to a protein of interest resulting in degradation of the fusion protein in the absence of stabilizing ligand. These engineered protein domains enable rapid, reversible and dose-dependent control of protein expression levels in cultured cells and in vivo. To broaden the scope of this technology, we have engineered new destabilizing domains that perform well at temperatures of 20-25°C. This raises the possibility that our technology could be adapted for use at any temperature. We further show that these new destabilizing domains can be used to regulate protein concentrations in C. elegans. These data reinforce that DD can function in virtually any organism and temperature.
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    • Grant Information:
      R01 AG025941 United States AG NIA NIH HHS; R01 GM073046 United States GM NIGMS NIH HHS; GM073046 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (Caenorhabditis elegans Proteins)
      EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase)
    • Publication Date:
      Date Created: 20130831 Date Completed: 20140615 Latest Revision: 20211021
    • Publication Date:
      20221213
    • Accession Number:
      PMC3750007
    • Accession Number:
      10.1371/journal.pone.0072393
    • Accession Number:
      23991108