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Full screening and accurate subtyping of HLA-A*02 alleles through group-specific amplification and mono-allelic sequencing.
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- Author(s): Song S; Han M; Zhang H; Wang Y; Jiang H
- Source:
Cellular & molecular immunology [Cell Mol Immunol] 2013 Nov; Vol. 10 (6), pp. 490-6. Date of Electronic Publication: 2013 Aug 19.- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Chinese Society of Immunology;; _b Nature Pub. Group Country of Publication: China NLM ID: 101242872 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2042-0226 (Electronic) Linking ISSN: 16727681 NLM ISO Abbreviation: Cell Mol Immunol Subsets: MEDLINE
- Publication Information: Original Publication: Beijing, China : Tokyo, Japan : Chinese Society of Immunology; Nature Pub. Group, 2010-
- Subject Terms: Alleles* ; Genetic Testing* ; Histocompatibility Testing*; HLA-A2 Antigen/*genetics ; Polymerase Chain Reaction/*methods ; Sequence Analysis, DNA/*methods; Base Sequence ; China ; Computational Biology ; DNA/genetics ; Exons/genetics ; Genome, Human/genetics ; Haploidy ; Humans ; Reference Standards ; Reproducibility of Results
- Abstract: HLA-A*02 is the most prevalent and polymorphic major histocompatibility complex (MHC) allele family in humans. Functional differences have been revealed among subtypes, demanding further subtyping of HLA-A*02 in basic and clinical settings. However, the fast growing polymorphisms render traditional primer- or probe-based typing methods impractical and result in increasing ambiguities in direct sequence-based typing. In this study, we combined group-specific amplification and mono-allelic sequencing to design and validate a simple scheme for the complete screening and accurate subtyping of all 540 reported HLA-A*02 alleles. This scheme could be performed in routine labs to facilitate studies with an interest in HLA-A*02.
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Methods Mol Biol. 2003;210:191-222. (PMID: 12412456) - Accession Number: 0 (HLA-A*02 antigen)
0 (HLA-A2 Antigen)
9007-49-2 (DNA) - Publication Date: Date Created: 20130820 Date Completed: 20140710 Latest Revision: 20211021
- Publication Date: 20231215
- Accession Number: PMC4002391
- Accession Number: 10.1038/cmi.2013.33
- Accession Number: 23954948
- Source:
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