Serotype-specific anti-Dengue virus NS1 mouse antibodies cross-react with prM and are potentially involved in virus production.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Additional Information
    • Source:
      Publisher: Mary Ann Liebert, Inc Country of Publication: United States NLM ID: 8801552 Publication Model: Print Cited Medium: Internet ISSN: 1557-8976 (Electronic) Linking ISSN: 08828245 NLM ISO Abbreviation: Viral Immunol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, NY : Mary Ann Liebert, Inc., c1987-
    • Subject Terms:
      Antibodies, Viral/*immunology ; Dengue Virus/*immunology ; Viral Envelope Proteins/*immunology ; Viral Nonstructural Proteins/*immunology; Aedes ; Animals ; Antibodies, Monoclonal/immunology ; Antibodies, Viral/blood ; Cells, Cultured ; Chlorocebus aethiops ; Cricetinae ; Cross Reactions/immunology ; Dengue/immunology ; Dengue/prevention & control ; HEK293 Cells ; Humans ; Mice ; Mice, Inbred BALB C ; Vero Cells ; Virus Replication/immunology
    • Abstract:
      Dengue virus (DENV) infection induces a strong B-cell immune response against the viral nonstructural protein 1 (NS1). Anti-NS1 antibodies (Abs) may affect virus production because they coexist with the virus in the patients' blood. The present study examined whether ten mouse monoclonal antibodies (MAbs) raised against NS1 affected production of the DENV-2. Three MAbs, 4C2, 4G11, and 4E5, showed weak neutralizing activity in a focus reduction assay. In addition, two serotype-specific MAbs, 4C2 and 4G11, protected suckling mice from lethal infection with DENV-2. An immunoprecipitation assay with DENV-2 showed that these MAbs, which were specific for the NS1 of DENV-4 and DENV-1, cross-reacted with the DENV-2 pre-membrane (prM) protein, but not with DENV-2 NS1. Interestingly, high concentrations of MAb 4G11 showed antibody-dependent enhancement of DENV-2 infection in human monocyte THP-1 cells. Taken together, these observations suggest that serotype-specific anti-NS1 MAbs are potentially involved in virus production.
    • Accession Number:
      0 (Antibodies, Monoclonal)
      0 (Antibodies, Viral)
      0 (NS1 protein, Dengue virus type 2)
      0 (NS1 protein, dengue-1 virus)
      0 (Viral Envelope Proteins)
      0 (Viral Nonstructural Proteins)
      0 (prM protein, Flavivirus)
    • Publication Date:
      Date Created: 20130815 Date Completed: 20140331 Latest Revision: 20191210
    • Publication Date:
      20240829
    • Accession Number:
      10.1089/vim.2012.0102
    • Accession Number:
      23941673