Epidermal growth factor-like domain 8 inhibits the survival and proliferation of mouse thymocytes.

Publisher: D.A. Spandidos Country of Publication: Greece NLM ID: 9810955 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1791-244X (Electronic) Linking ISSN: 11073756 NLM ISO Abbreviation: Int J Mol Med Subsets: MEDLINE

Original Publication: Athens, Greece : D.A. Spandidos, [1998-

Cell Proliferation*; Cell Survival/*physiology ; Proteins/*metabolism ; Thymocytes/*metabolism; Animals ; Apoptosis ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Basic Helix-Loop-Helix Transcription Factors/metabolism ; Calcium-Binding Proteins ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Cell Differentiation ; Cell Line ; Cloning, Molecular ; EGF Family of Proteins ; Epithelial Cells/cytology ; Epithelial Cells/metabolism ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Lymphocyte Activation ; Mice ; Organ Size ; Proteins/genetics ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Signal Transduction ; Thymocytes/pathology ; Transcription Factor HES-1

Thymic epithelial cells (TECs) play a critical role in T-cell development through their intercellular interactions and by producing various soluble proteins, such as growth factors, cytokines and chemokines. In this study, we report a new role for epidermal growth factor-like domain 8 (EGFL8) in the regulation of the survival and proliferation of mouse thymocytes. Mouse recombinant EGFL8 (rEGFL8) protein was produced using an E. coli system and its biological role in mouse thymocytes was determined. The injection of rEGFL8 in mice in vivo resulted in a decrease in the weight of the thymus, as well as in the number of total thymocytes; rEGFL8 also inhibited thymocyte proliferation and induced thymocyte apoptosis. Furthermore, rEGFL8 suppressed the expression of the Notch downstream targets, Hes1 and Hey1, in mouse thymocytes and TECs, indicating that EGFL8 negatively regulates the Notch signaling pathway in these cells. The identification of the role of EGFL8 in thymocytes may aid in the determination of the fate of thymocytes during T-cell development.

0 (Basic Helix-Loop-Helix Transcription Factors)
0 (Calcium-Binding Proteins)
0 (Cell Cycle Proteins)
0 (EGF Family of Proteins)
0 (Egfl8 protein, mouse)
0 (Hes1 protein, mouse)
0 (Hey1 protein, mouse)
0 (Homeodomain Proteins)
0 (Proteins)
0 (Recombinant Proteins)
0 (Transcription Factor HES-1)

Date Created: 20130724 Date Completed: 20140327 Latest Revision: 20201209

20221213

10.3892/ijmm.2013.1448

23877103