[Mitokorrektine stimulates angiogenesis in vitro].

Publisher: Naukova Dumka Country of Publication: Ukraine NLM ID: 9601541 Publication Model: Print Cited Medium: Print ISSN: 2522-9028 (Print) Linking ISSN: 25229028 NLM ISO Abbreviation: Fiziol Zh (1994) Subsets: MEDLINE

Publication: Kyiv : Naukova Dumka
Original Publication: Kyïv : Nauk. dumka, 1994-

Cell Proliferation/*drug effects ; Endothelial Cells/*drug effects ; Neovascularization, Physiologic/*drug effects ; Oligopeptides/*pharmacology; Animals ; Animals, Newborn ; Brain Chemistry ; Cell Cycle/drug effects ; Cell Differentiation/drug effects ; Cell Line ; Cell Movement/drug effects ; Cell Survival/drug effects ; Dose-Response Relationship, Drug ; Endothelial Cells/cytology ; Endothelial Cells/metabolism ; Flow Cytometry ; Glucose/metabolism ; Liver/chemistry ; Mitochondria/chemistry ; Nitric Oxide/metabolism ; Pancreas/chemistry ; Swine

The effect of mitokorrectine (complex native oligopeptides isolated from neonatal pig brain) on endothelial cells in culture was investigated. It was revealed that the drug concentration-dependently induces angiogenesis in vitro. Mitogen effect of Mitokorrectine was shown by MTT-test and routine cell count in concentration diapason (0.1-1 mg/ml) which means an increased number of cells by 25 +/- 5% and cell subpopulation of proliferative pool (G2/M+S) 1,8 times in concentration diapason mitokorrectine (0.01-0.05 mg/ml) in comparison with control. In 3-D culture and in stationary phase we detected induction of differentiation of endothelial cells, a decrease the level of NO production and enhancement of glucose metabolism and stimulation of formation of capillary-like tubes.

0 (Oligopeptides)
31C4KY9ESH (Nitric Oxide)
IY9XDZ35W2 (Glucose)

Date Created: 20130705 Date Completed: 20130729 Latest Revision: 20131121

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