Helicobacter pylori outer membrane protein HopQ identified as a novel T4SS-associated virulence factor.

Publisher: Hindawi Country of Publication: India NLM ID: 100883691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1462-5822 (Electronic) Linking ISSN: 14625814 NLM ISO Abbreviation: Cell Microbiol Subsets: MEDLINE

Publication: 2022- : Mumbai : Hindawi
Original Publication: Oxford : Wiley-Blackwell, c1999-

Bacterial Secretion Systems*; Bacterial Outer Membrane Proteins/*metabolism ; Helicobacter pylori/*metabolism ; Virulence Factors/*metabolism; Bacterial Outer Membrane Proteins/genetics ; Cell Line ; DNA Transposable Elements ; Epithelial Cells/microbiology ; Gene Deletion ; Helicobacter pylori/genetics ; Helicobacter pylori/pathogenicity ; Humans ; Interleukin-8/metabolism ; Mitogen-Activated Protein Kinase Kinases/metabolism ; Molecular Sequence Data ; Mutagenesis, Insertional ; NF-kappa B/metabolism ; Sequence Analysis, DNA ; Signal Transduction ; Virulence Factors/genetics

Helicobacter pylori is a bacterial pathogen that colonizes the gastric niche of ∼ 50% of the human population worldwide and is known to cause peptic ulceration and gastric cancer. Pathology of infection strongly depends on a cag pathogenicity island (cagPAI)-encoded type IV secretion system (T4SS). Here, we aimed to identify as yet unknown bacterial factors involved in cagPAI effector function and performed a large-scale screen of an H. pylori transposon mutant library using activation of the pro-inflammatory transcription factor NF-κB in human gastric epithelial cells as a measure of T4SS function. Analysis of ∼ 3000 H. pylori mutants revealed three non-cagPAI genes that affected NF-κB nuclear translocation. Of these, the outer membrane protein HopQ from H. pylori strain P12 was essential for CagA translocation and for CagA-mediated host cell responses such as formation of the hummingbird phenotype and cell scattering. Besides that, deletion of hopQ reduced T4SS-dependent activation of NF-κB, induction of MAPK signalling and secretion of interleukin 8 (IL-8) in the host cells, but did not affect motility or the quantity of bacteria attached to host cells. Hence, we identified HopQ as a non-cagPAI-encoded cofactor of T4SS function.
(© 2013 John Wiley & Sons Ltd.)

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R01 AI054423 United States AI NIAID NIH HHS

GENBANK ACJ08294; JF303025

0 (Bacterial Outer Membrane Proteins)
0 (Bacterial Secretion Systems)
0 (DNA Transposable Elements)
0 (Interleukin-8)
0 (NF-kappa B)
0 (Virulence Factors)
EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)

Date Created: 20130621 Date Completed: 20140506 Latest Revision: 20211021

20221213

PMC3797234

10.1111/cmi.12158

23782461