Curcumin inhibits AP-2γ-induced apoptosis in the human malignant testicular germ cells in vitro.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 100956087 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1745-7254 (Electronic) Linking ISSN: 16714083 NLM ISO Abbreviation: Acta Pharmacol Sin Subsets: MEDLINE
    • Publication Information:
      Publication: 2009- : New York : Nature Publishing Group
      Original Publication: Beijing, China : Science Press, c2000-
    • Subject Terms:
      Neoplasms, Germ Cell and Embryonal*/drug therapy ; Neoplasms, Germ Cell and Embryonal*/pathology ; Testicular Neoplasms*/drug therapy ; Testicular Neoplasms*/pathology; Antineoplastic Agents/*pharmacology ; Apoptosis/*drug effects ; Cell Proliferation/*drug effects ; Curcumin/*pharmacology ; Transcription Factor AP-2/*antagonists & inhibitors ; Transcription Factor AP-2/*pharmacology; Animals ; Antineoplastic Agents/therapeutic use ; Apoptosis/physiology ; Curcumin/therapeutic use ; Humans ; Mice
    • Abstract:
      Aim: To investigate the effects of curcumin on proliferation and apoptosis in testicular cancer cells in vitro and to investigate its molecular mechanisms of action.
      Methods: NTera-2 human malignant testicular germ cell line and F9 mouse teratocarcinoma stem cell line were used. The anti-proliferative effect was examined using MTT and colony formation assays. Hoechst 33258 staining, TUNEL and Annexin V-FITC/PI staining assays were used to analyze cell apoptosis. Protein expression was examined with Western blot analysis and immunocytochemical staining.
      Results: Curcumin (5, 10 and 15 μmol/L) inhibited the viability of NTera-2 cells in dose- and time-dependent manners. Curcumin significantly inhibited the colony formation in both NTera-2 and F9 cells. Curcumin dose-dependently induced apoptosis of NTera-2 cells by reducing FasL expression and Bcl-2-to-Bax ratio, and activating caspase-9, -8 and -3. Furthermore, curcumin dose-dependently reduced the expression of AP transcription factor AP-2γ in NTera-2 cells, whereas the pretreatment with the proteasome inhibitor MG132 blocked both the curcumin-induced reduction of AP-2γ and antiproliferative effect. Curcumin inhibited ErbB2 expression, and decreased the phosphorylation of Akt and ERK in NTera-2 cells.
      Conclusion: Curcumin induces apoptosis and inhibits proliferation in NTera-2 cells via the inhibition of AP-2γ-mediated downstream cell survival signaling pathways.
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    • Accession Number:
      0 (Antineoplastic Agents)
      0 (TFAP2C protein, human)
      0 (Transcription Factor AP-2)
      IT942ZTH98 (Curcumin)
    • Subject Terms:
      Testicular Germ Cell Tumor
    • Publication Date:
      Date Created: 20130521 Date Completed: 20140714 Latest Revision: 20220316
    • Publication Date:
      20231215
    • Accession Number:
      PMC4003153
    • Accession Number:
      10.1038/aps.2013.38
    • Accession Number:
      23685957