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Curcumin inhibits AP-2γ-induced apoptosis in the human malignant testicular germ cells in vitro.
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- Author(s): Zhou C;Zhou C; Zhao XM; Li XF; Wang C; Zhang XT; Liu XZ; Ding XF; Xiang SL; Zhang J
- Source:
Acta pharmacologica Sinica [Acta Pharmacol Sin] 2013 Sep; Vol. 34 (9), pp. 1192-200. Date of Electronic Publication: 2013 May 20.- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 100956087 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1745-7254 (Electronic) Linking ISSN: 16714083 NLM ISO Abbreviation: Acta Pharmacol Sin Subsets: MEDLINE
- Publication Information: Publication: 2009- : New York : Nature Publishing Group
Original Publication: Beijing, China : Science Press, c2000- - Subject Terms: Neoplasms, Germ Cell and Embryonal*/drug therapy ; Neoplasms, Germ Cell and Embryonal*/pathology ; Testicular Neoplasms*/drug therapy ; Testicular Neoplasms*/pathology; Antineoplastic Agents/*pharmacology ; Apoptosis/*drug effects ; Cell Proliferation/*drug effects ; Curcumin/*pharmacology ; Transcription Factor AP-2/*antagonists & inhibitors ; Transcription Factor AP-2/*pharmacology; Animals ; Antineoplastic Agents/therapeutic use ; Apoptosis/physiology ; Curcumin/therapeutic use ; Humans ; Mice
- Abstract: Aim: To investigate the effects of curcumin on proliferation and apoptosis in testicular cancer cells in vitro and to investigate its molecular mechanisms of action.
Methods: NTera-2 human malignant testicular germ cell line and F9 mouse teratocarcinoma stem cell line were used. The anti-proliferative effect was examined using MTT and colony formation assays. Hoechst 33258 staining, TUNEL and Annexin V-FITC/PI staining assays were used to analyze cell apoptosis. Protein expression was examined with Western blot analysis and immunocytochemical staining.
Results: Curcumin (5, 10 and 15 μmol/L) inhibited the viability of NTera-2 cells in dose- and time-dependent manners. Curcumin significantly inhibited the colony formation in both NTera-2 and F9 cells. Curcumin dose-dependently induced apoptosis of NTera-2 cells by reducing FasL expression and Bcl-2-to-Bax ratio, and activating caspase-9, -8 and -3. Furthermore, curcumin dose-dependently reduced the expression of AP transcription factor AP-2γ in NTera-2 cells, whereas the pretreatment with the proteasome inhibitor MG132 blocked both the curcumin-induced reduction of AP-2γ and antiproliferative effect. Curcumin inhibited ErbB2 expression, and decreased the phosphorylation of Akt and ERK in NTera-2 cells.
Conclusion: Curcumin induces apoptosis and inhibits proliferation in NTera-2 cells via the inhibition of AP-2γ-mediated downstream cell survival signaling pathways. - References: Cancer Lett. 2008 Aug 18;267(1):133-64. (PMID: 18462866)
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Neoplasia. 2006 Jul;8(7):568-77. (PMID: 16867219) - Accession Number: 0 (Antineoplastic Agents)
0 (TFAP2C protein, human)
0 (Transcription Factor AP-2)
IT942ZTH98 (Curcumin) - Subject Terms: Testicular Germ Cell Tumor
- Publication Date: Date Created: 20130521 Date Completed: 20140714 Latest Revision: 20220316
- Publication Date: 20231215
- Accession Number: PMC4003153
- Accession Number: 10.1038/aps.2013.38
- Accession Number: 23685957
- Source:
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