CD271 defines a stem cell-like population in hypopharyngeal cancer.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Gene Expression Regulation, Neoplastic*; Biomarkers, Tumor/*genetics ; Carcinoma, Squamous Cell/*genetics ; Hypopharyngeal Neoplasms/*genetics ; Hypopharynx/*metabolism ; Neoplastic Stem Cells/*metabolism ; Nerve Tissue Proteins/*genetics ; Receptors, Nerve Growth Factor/*genetics; Animals ; Antineoplastic Agents/pharmacology ; Biomarkers, Tumor/metabolism ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/mortality ; Carcinoma, Squamous Cell/pathology ; Cisplatin/pharmacology ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Humans ; Hypopharyngeal Neoplasms/metabolism ; Hypopharyngeal Neoplasms/mortality ; Hypopharyngeal Neoplasms/pathology ; Hypopharynx/pathology ; Matrix Metalloproteinases/genetics ; Matrix Metalloproteinases/metabolism ; Mice ; Mice, Inbred NOD ; Mice, SCID ; Nanog Homeobox Protein ; Neoplasm Staging ; Neoplasm Transplantation ; Neoplastic Stem Cells/pathology ; Nerve Tissue Proteins/metabolism ; Receptors, Nerve Growth Factor/metabolism ; Survival Analysis ; Transplantation, Heterologous ; Tumor Cells, Cultured

Cancer stem cells contribute to the malignant phenotypes of a variety of cancers, but markers to identify human hypopharyngeal cancer (HPC) stem cells remain poorly understood. Here, we report that the CD271(+) population sorted from xenotransplanted HPCs possesses an enhanced tumor-initiating capability in immunodeficient mice. Tumors generated from the CD271(+) cells contained both CD271(+) and CD271(-) cells, indicating that the population could undergo differentiation. Immunohistological analyses of the tumors revealed that the CD271(+) cells localized to a perivascular niche near CD34(+) vasculature, to invasive fronts, and to the basal layer. In accordance with these characteristics, a stemness marker, Nanog, and matrix metalloproteinases (MMPs), which are implicated in cancer invasion, were significantly up-regulated in the CD271(+) compared to the CD271 (-) cell population. Furthermore, using primary HPC specimens, we demonstrated that high CD271 expression was correlated with a poor prognosis for patients. Taken together, our findings indicate that CD271 is a novel marker for HPC stem-like cells and for HPC prognosis.

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0 (Antineoplastic Agents)
0 (Biomarkers, Tumor)
0 (Homeodomain Proteins)
0 (NANOG protein, human)
0 (NGFR protein, human)
0 (Nanog Homeobox Protein)
0 (Nerve Tissue Proteins)
0 (Receptors, Nerve Growth Factor)
EC 3.4.24.- (Matrix Metalloproteinases)
Q20Q21Q62J (Cisplatin)

Date Created: 20130430 Date Completed: 20131126 Latest Revision: 20231106

20240829

PMC3633921

10.1371/journal.pone.0062002

23626764