Ultrastructural changes in the air-blood barrier in mice after intratracheal instillations of Asian sand dust and gold nanoparticles.

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  • Additional Information
    • Source:
      Publisher: Elsevier GmbH Country of Publication: Germany NLM ID: 9208920 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1618-1433 (Electronic) Linking ISSN: 09402993 NLM ISO Abbreviation: Exp Toxicol Pathol Subsets: MEDLINE
    • Publication Information:
      Publication: [Munich] : Elsevier GmbH
      Original Publication: Jena ; New York : G. Fischer, c1992-
    • Subject Terms:
      Dust*; Blood-Air Barrier/*ultrastructure ; Gold/*toxicity ; Nanoparticles/*toxicity ; Silicon Dioxide/*toxicity; Air Pollutants/toxicity ; Animals ; Asia ; Bronchoalveolar Lavage Fluid/chemistry ; Endothelium, Vascular/ultrastructure ; Immunohistochemistry ; Male ; Mice ; Mice, Inbred ICR ; Microscopy, Electron, Transmission
    • Abstract:
      The purpose of this study was to investigate a possible translocation pathway of intratracheally instilled gold nanoparticles after the induction of acute pulmonary injury by Asian sand dust. ICR mice were intratracheally instilled with 800μg Asian sand particles (CJ-2 particles) 24h before instillation of 50-nm gold nanoparticles. Lungs from mice treated with Asian sand particles and gold nanoparticles showed an acute focal inflammation with an increased expression of proinflammatory cytokines (IL-6 and TNF-α) and oxidative stress markers (Cu/Zn SOD and iNOS) in alveolar macrophages, type I alveolar epithelial cells, and endothelial cells at the alveolar walls. Electron microscopy revealed a destruction of the alveolar walls with an increased number of endocytic vesicles in the cytoplasm of both type I epithelial cells and endothelial cells; gold nanoparticles were demonstrated in these endocytic vesicles. These findings suggest that translocation of the exposed nanoparticles may be enhanced in the lung tissues with acute inflammatory changes.
      (Copyright © 2013 Elsevier GmbH. All rights reserved.)
    • Contributed Indexing:
      Keywords: Air–blood barrier; Cu/Zn SOD; IL-6; Kosa; Laminin; TNF-α; Translocation pathway; iNOS
    • Accession Number:
      0 (Air Pollutants)
      0 (Dust)
      7440-57-5 (Gold)
      7631-86-9 (Silicon Dioxide)
    • Publication Date:
      Date Created: 20130430 Date Completed: 20140607 Latest Revision: 20131021
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/j.etp.2013.03.003
    • Accession Number:
      23623190