Changes in the Expression of Transcription Factors Involved in Modulating the Expression of EPO-R in Activated Human CD4-Positive Lymphocytes.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Lymphocyte Activation*; CD4-Positive T-Lymphocytes/*immunology ; CD4-Positive T-Lymphocytes/*metabolism ; Gene Expression Regulation/*immunology ; Receptors, Erythropoietin/*genetics ; Transcription Factors/*metabolism; Adult ; Antibodies, Monoclonal/immunology ; CD3 Complex/immunology ; Female ; Humans ; Male

We have recently described the presence of the erythropoietin receptor (EPO-R) on CD4(+) lymphocytes and demonstrated that its expression increases during their activation, reaching a level reported to be typical for erythroid progenitors. This observation suggests that EPO-R expression is modulated during lymphocyte activation, which may be important for the cells' function. Here we investigated whether the expression of GATA1, GATA3 and Sp1 transcription factors is correlated with the expression of EPO-R in human CD4(+) lymphocytes stimulated with monoclonal anti-CD3 antibody. The expression of GATA1, GATA3 and Sp1 transcription factors in CD4(+) cells was estimated before and after stimulation with anti-CD3 antibody by Western Blot and flow cytometry. The expression of EPO-R was measured using real-time PCR and flow cytometry. There was no change in the expression of GATA1 and GATA3 in CD4(+) lymphocytes after stimulation with anti-CD3 antibody. However, stimulation resulted in the significantly increased expression of the Sp1 factor. CD4(+) lymphocytes stimulated with anti-CD3 antibody exhibited an increase in both the expression level of EPOR gene and the number of EPO-R molecules on the cells' surface, the latter being significantly correlated with the increased expression of Sp1. Sp1 is noted to be the single transcription factor among the ones studied whose level changes as a result of CD4(+) lymphocyte stimulation. It seems that Sp1 may significantly affect the number of EPO-R molecules present on the surface of activated CD4(+) lymphocytes.

Nephron. 2001 Jul;88(3):205-10. (PMID: 11423750)
J Clin Immunol. 2013 Apr;33(3):661-5. (PMID: 23180360)
Mol Cell Biol. 1993 Jul;13(7):4011-22. (PMID: 8321208)
Blood. 2003 Nov 15;102(10):3575-83. (PMID: 12893747)
Pediatr Res. 2009 Jun;65(6):619-24. (PMID: 19218878)
J Recept Signal Transduct Res. 2011 Jun;31(3):241-6. (PMID: 21619451)
Blood. 1992 Oct 15;80(8):1940-9. (PMID: 1391953)
Artif Organs. 1998 Mar;22(3):177-81. (PMID: 9527275)
Cell. 1997 May 16;89(4):587-96. (PMID: 9160750)
Blood. 2005 Nov 15;106(10):3440-8. (PMID: 16091457)
J Biol Chem. 2002 Mar 15;277(11):9598-605. (PMID: 11779871)
Nephrol Dial Transplant. 2002 Jun;17(6):1070-80. (PMID: 12032199)
Cell Mol Immunol. 2007 Feb;4(1):15-29. (PMID: 17349208)
J Biol Chem. 1987 Oct 15;262(29):13985-90. (PMID: 2820989)
Nucleic Acids Res. 1995 Aug 11;23(15):3041-9. (PMID: 7659529)
Artif Organs. 2010 Aug;34(8):654-62. (PMID: 20528849)
Proc Natl Acad Sci U S A. 1990 Aug;87(15):5978-82. (PMID: 2165612)
Artif Organs. 1999 Sep;23(9):809-16. (PMID: 10491027)
EMBO J. 1991 May;10(5):1187-92. (PMID: 1827068)
Artif Organs. 2010 Mar;34(3):E77-84. (PMID: 20447038)
J Biol Chem. 2000 Dec 15;275(50):39754-61. (PMID: 10995753)
Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10638-41. (PMID: 1660143)
Blood. 2006 Jan 1;107(1):87-97. (PMID: 16144799)
J Cell Mol Med. 2011 Sep;15(9):1963-72. (PMID: 21029371)

0 (Antibodies, Monoclonal)
0 (CD3 Complex)
0 (Receptors, Erythropoietin)
0 (Transcription Factors)

Date Created: 20130412 Date Completed: 20131022 Latest Revision: 20211021

20250114

PMC3618423

10.1371/journal.pone.0060326

23577103