Emodin improves lipopolysaccharide-induced microcirculatory disturbance in rat mesentery.

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  • Additional Information
    • Source:
      Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 9434935 Publication Model: Print Cited Medium: Internet ISSN: 1549-8719 (Electronic) Linking ISSN: 10739688 NLM ISO Abbreviation: Microcirculation Subsets: MEDLINE
    • Publication Information:
      Publication: Malden, MA : Wiley-Blackwell
      Original Publication: New York, NY : Chapman & Hall, c1994-
    • Subject Terms:
      Mesentery*/blood supply ; Mesentery*/metabolism ; Mesentery*/pathology ; Mesentery*/physiopathology; Emodin/*pharmacology ; Lipopolysaccharides/*toxicity ; Microcirculation/*drug effects ; Protein Kinase Inhibitors/*pharmacology ; Regional Blood Flow/*drug effects; Animals ; Endotoxemia/chemically induced ; Endotoxemia/drug therapy ; Endotoxemia/metabolism ; Endotoxemia/physiopathology ; Humans ; Intercellular Adhesion Molecule-1/biosynthesis ; Male ; Peroxidase/biosynthesis ; Rats ; Rats, Wistar ; Toll-Like Receptor 4/biosynthesis ; Transcription Factor AP-1/biosynthesis ; Transcription Factor RelA/biosynthesis
    • Abstract:
      Objective: Sepsis is a systemic inflammatory response syndrome. Emodin is a major ingredient of Rheum Palmatum, a Chinese herb that is widely used in China for treatment of endotoxemia-related diseases. This study intended to examine the effect of Emodin on LPS-induced rat mesenteric microcirculatory disturbance and the underlying mechanisms.
      Methods: The male Wistar rats received LPS (5 mg/kg/hr) for 90 min, with or without administration of Emodin (10 mg/kg/hr) by enema 30 min before (pre-treatment) or after (post-treatment) LPS infusion, and the dynamics of mesenteric microcirculation were determined by inverted intravital microscopy. Expression of adhesion molecules and TLR4, NF-κB p65, ICAM-1, MPO, and AP-1 in mesentery tissue was evaluated by flow cytometry and Western-blot, respectively.
      Results: Pre or post-treatment with Emodin significantly ameliorated LPS-induced leukocyte emigration, reactive oxygen species production and albumin leakage, and the expression of TLR4, NF-κB p65, ICAM-1, MPO and AP-1 in mesentery.
      Conclusions: These results demonstrate the beneficial role of Emodin in attenuating the LPS-induced microcirculatory disturbance, and support the use of Emodin for patients with endotoxemia.
      (© 2013 John Wiley & Sons Ltd.)
    • Contributed Indexing:
      Keywords: Rheum palmatum; activator protein-1; adhesion molecules; nuclear factor kappa B p65; toll-like receptor 4
    • Accession Number:
      0 (Lipopolysaccharides)
      0 (Protein Kinase Inhibitors)
      0 (Rela protein, rat)
      0 (Tlr4 protein, rat)
      0 (Toll-Like Receptor 4)
      0 (Transcription Factor AP-1)
      0 (Transcription Factor RelA)
      126547-89-5 (Intercellular Adhesion Molecule-1)
      EC 1.11.1.7 (Peroxidase)
      KA46RNI6HN (Emodin)
    • Publication Date:
      Date Created: 20130405 Date Completed: 20140430 Latest Revision: 20131014
    • Publication Date:
      20231215
    • Accession Number:
      10.1111/micc.12061
    • Accession Number:
      23551520