Repair of an articular cartilage defect using adipose-derived stem cells loaded on a polyelectrolyte complex scaffold based on poly(l-glutamic acid) and chitosan.

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  • Author(s): Zhang K;Zhang K; Zhang Y; Yan S; Gong L; Wang J; Chen X; Cui L; Yin J
  • Source:
    Acta biomaterialia [Acta Biomater] 2013 Jul; Vol. 9 (7), pp. 7276-88. Date of Electronic Publication: 2013 Mar 25.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: England NLM ID: 101233144 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-7568 (Electronic) Linking ISSN: 17427061 NLM ISO Abbreviation: Acta Biomater Subsets: MEDLINE
    • Publication Information:
      Original Publication: Kidlington, Oxford, UK : Elsevier, c2004-
    • Subject Terms:
      Tissue Scaffolds*; Chitosan/*chemistry ; Fractures, Cartilage/*surgery ; Polyglutamic Acid/*chemistry ; Stem Cell Transplantation/*instrumentation ; Stem Cells/*cytology; Adipose Tissue/cytology ; Animals ; Cell Differentiation ; Cells, Cultured ; Chondrogenesis ; Electrolytes/chemistry ; Fracture Healing ; Fractures, Cartilage/pathology ; Rabbits ; Treatment Outcome
    • Abstract:
      As a synthetic polypeptide water-soluble poly(l-glutamic acid) (PLGA) was designed to fabricate scaffolds for cartilage tissue engineering. Chitosan (CHI) has been employed as a physical cross-linking component in the construction of scaffolds. PLGA/CHI scaffolds act as sponges with a swelling ratio of 760±45% (mass%), showing promising biocompatibility and biodegradation. Autologous adipose-derived stem cells (ASCs) were expanded and seeded on PLGA/CHI scaffolds, ASC/scaffold constructs were then subjected to chondrogenic induction in vitro for 2weeks. The results showed that PLGA/CHI scaffolds could effectively support ASC adherence, proliferation and chondrogenic differentiation. The ASCs/scaffold constructs were then transplanted to repair full thickness articular cartilage defects (4mm in diameter, to the depth of subchondral bone) created in rabbit femur trochlea. Histological observations found that articular defects were covered with newly formed cartilage 6weeks post-implantation. After 12weeks the regenerated cartilage had integrated well with the surrounding native cartilage and subchondral bone. Toluidine blue and immunohistochemical staining confirmed similar accumulation of glycosaminoglycans and type II collagen in engineered cartilage as in native cartilage 12weeks post-implantation. The result was further supported by quantitative analysis of extracellular matrix deposition. The compressive modulus of the engineered cartilage increased significantly from 30% of that of normal cartilage at 6weeks to 83% at 12weeks. Cyto-nanoindentation also showed analogous biomechanical behavior of the engineered cartilage to that of native cartilage. The results of the present study thus demonstrate the potentiality of PLGA/CHI scaffolds in cartilage tissue engineering.
      (Copyright © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
    • Accession Number:
      0 (Electrolytes)
      25513-46-6 (Polyglutamic Acid)
      9012-76-4 (Chitosan)
    • Publication Date:
      Date Created: 20130329 Date Completed: 20131017 Latest Revision: 20220330
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.actbio.2013.03.025
    • Accession Number:
      23535234