[Characteristic of fluoroquinolone resistant clinical isolates of K. pneumoniae, P. mirabilis and E. coli producing ESBL and AmpC beta-lactamases].

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  • Additional Information
    • Transliterated Title:
      Charakterystyka opornych na fluorochinolony izolatów z gatunku K. pneumoniae, P. mirabilis i E. coli wytwarzajacych beta - laktamazy typu ESBL i AmpC.
    • Source:
      Publisher: Panstwowy Zaklad Wydawnictw Lekarskich Country of Publication: Poland NLM ID: 0210575 Publication Model: Print Cited Medium: Print ISSN: 0025-8601 (Print) Linking ISSN: 00258601 NLM ISO Abbreviation: Med Dosw Mikrobiol Subsets: MEDLINE
    • Publication Information:
      Publication: Warszawa : Panstwowy Zaklad Wydawnictw Lekarskich
      Original Publication: [Warszawa, Lekarski Instytut Naukowo-Wydawniczy]
    • Subject Terms:
      Drug Resistance, Bacterial/*genetics ; Escherichia coli/*metabolism ; Fluoroquinolones/*pharmacology ; Klebsiella pneumoniae/*metabolism ; Plasmids/*metabolism ; Proteus mirabilis/*metabolism ; beta-Lactamases/*biosynthesis; Bacterial Proteins/biosynthesis ; Cephalosporins/pharmacology ; Escherichia coli/genetics ; Klebsiella pneumoniae/genetics ; Microbial Sensitivity Tests ; Proteus mirabilis/genetics ; beta-Lactam Resistance/genetics
    • Abstract:
      Introduction: Extended-spectrum beta-lactamases (ESBLs) are the predominant mechanism for acquired antibiotic resistance in Enterobacteriaceae. During the past few years, increasing occurrence of plasmid-mediated AmpC beta-lactamases (pAmpCs) particularly in K. pneumonia, P. mirabilis and E. coli was reported. Therefore, the aim of our study was to analyse of the diversity of plasmid-mediated beta-lactamases such as pAmpCs and ESBLs among clinical K. pneumonia, P. mirabilis and E. coli strains in Poland.
      Methods: A total of 19 clinical Enterobacteriaceae strains (E. coli, n = 9; K. pneumoniae, n = 7; P. mirabilis, n = 3) resistant to third-generation cephalosporin were selected from collection of fluoroquinolone resistant isolates recovered during a 6-months period in regular hospital in Warsaw, Poland. ESBLs and AmpCs were detected by using phenotypic methods: double-disc tests (DDSTs), MAST ID D68C test, sensitivity to cefoxitin, disk potentiation test (DPT) and Tris-EDTA test. Polymerase chain reaction (PCR) amplification of the bla(AmpC), bla(CTM-M), bla(TEM), and bla(SHV), genes. PCR-products for these genes were sequenced. To determine the possible clonality of the tested isolates PFGE with the XbaI was performed.
      Results: Nine of 19 fluoroquinolone-resistant strains tested produced extended-spectrum beta-lactamases of TEM, SHV and CTX-M families. These ESBLs were most commonly detected in E. coli. AmpC beta-lactamases were produced by 6 tested strains, including 3 isolates of P. mirabilis. The AmpC found in our study belonged to CMY and DHA families, Furthermore, 4 isolates of K. pneumoniae were found to co-produce both ESBL and AmpC beta-lactamases. XbaI-PFGE profiles pointed significant differences of tested strains.
      Conclusion: Horizontal transfer of genes encoding for acquired beta-lactamases such ESBL and AmpC seem to play primary role in dissemination of these resistance traits among fluoroquinolone-resistant clinical strains of Enterobacteriaceae in Poland.
    • Accession Number:
      0 (Bacterial Proteins)
      0 (Cephalosporins)
      0 (Fluoroquinolones)
      EC 3.5.2.6 (AmpC beta-lactamases)
      EC 3.5.2.6 (beta-Lactamases)
    • Publication Date:
      Date Created: 20130315 Date Completed: 20130404 Latest Revision: 20220408
    • Publication Date:
      20231215
    • Accession Number:
      23484420