De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE

Original Publication: San Francisco, CA : Public Library of Science

Transcriptome*; Curcuma/*chemistry ; Curcuma/*genetics ; Plant Extracts/*chemistry ; Rhizome/*chemistry ; Terpenes/*pharmacology; Antimalarials/pharmacology ; Antineoplastic Agents/pharmacology ; Cluster Analysis ; Computational Biology ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Microsatellite Repeats ; Molecular Sequence Annotation ; Sequence Analysis, DNA

Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

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0 (Antimalarials)
0 (Antineoplastic Agents)
0 (Plant Extracts)
0 (Terpenes)
856YO1Z64F (turmeric extract)

Date Created: 20130308 Date Completed: 20130823 Latest Revision: 20211021

20231215

PMC3585318

10.1371/journal.pone.0056217

23468859