5-Hydroxymethylfurfural induces ADH7 and ARI1 expression in tolerant industrial Saccharomyces cerevisiae strain P6H9 during bioethanol production.

Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE

Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-

Adaptation, Physiological/*drug effects ; Alcohol Dehydrogenase/*genetics ; Aldehyde Reductase/*genetics ; Biofuels/*microbiology ; Ethanol/*metabolism ; Furaldehyde/*analogs & derivatives ; Saccharomyces cerevisiae/*enzymology ; Saccharomyces cerevisiae Proteins/*genetics; Alcohol Dehydrogenase/metabolism ; Aldehyde Reductase/metabolism ; Biomass ; Enzyme Induction/drug effects ; Furaldehyde/pharmacology ; Gene Expression Regulation, Enzymologic/drug effects ; Gene Expression Regulation, Fungal/drug effects ; Genetic Engineering ; Industrial Microbiology ; Kinetics ; Saccharomyces cerevisiae/drug effects ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/growth & development ; Saccharomyces cerevisiae Proteins/metabolism

The aims of this work were to obtain, by evolutionary engineering, an industrial strain of Saccharomyces cerevisiae tolerant to high concentrations of HMF and to determine the expression levels of genes previously described as responsible for this tolerance. Cells were grown under anaerobic and oxygen limited conditions, in the presence of glucose or sucrose as carbon sources. P6H9 strain presented high expression levels for genes ADH7 and ARI1 in presence of HMF. This tolerant strain also showed higher ethanol productivity, biomass formation and alcohol dehydrogenase activity comparing to sensitive strains. Results suggest that S. cerevisiae P6H9 strain presents potential to be used for second-generation ethanol production.
(Copyright © 2013 Elsevier Ltd. All rights reserved.)

0 (Biofuels)
0 (Saccharomyces cerevisiae Proteins)
3K9958V90M (Ethanol)
70ETD81LF0 (5-hydroxymethylfurfural)
DJ1HGI319P (Furaldehyde)
EC 1.1.1.1 (ADH7 protein, S cerevisiae)
EC 1.1.1.1 (Alcohol Dehydrogenase)
EC 1.1.1.21 (Aldehyde Reductase)
EC 1.1.1.21 (Ari1 protein, S cerevisiae)

Date Created: 20130221 Date Completed: 20131017 Latest Revision: 20131121

20240829

10.1016/j.biortech.2013.01.063

23422309