Development and application of nanoparticles synthesized with folic acid conjugated soy protein.

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  • Author(s): Teng Z;Teng Z; Luo Y; Wang T; Zhang B; Wang Q
  • Source:
    Journal of agricultural and food chemistry [J Agric Food Chem] 2013 Mar 13; Vol. 61 (10), pp. 2556-64. Date of Electronic Publication: 2013 Feb 26.
  • Publication Type:
    Evaluation Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
    • Publication Information:
      Original Publication: Washington, American Chemical Society.
    • Subject Terms:
      Drug Carriers/*chemistry ; Drug Delivery Systems/*methods ; Folic Acid/*chemistry ; Nanoparticles/*chemistry ; Soybean Proteins/*chemistry; Caco-2 Cells ; Curcumin/chemistry ; Curcumin/pharmacokinetics ; Drug Delivery Systems/instrumentation ; Humans ; Particle Size
    • Abstract:
      In this study, soy protein isolate (SPI) was conjugated with folic acid (FA) to prepare nanoparticles for target-specific drug delivery. Successful conjugation was evidenced by UV spectrophotometry and primary amino group analysis. An increase in count rate by at least 142% was observed in FA-SPI nanoparticles compared to the nonconjugated ones, whereas the particle size was decreased upon FA conjugation. This was probably attributed to the substitution of positively charged lysine residues by the FA backbone. The ζ-potential ranged from -36 to -42 mV depending on the conjugation degree, indicating desirable dispersion stability. Curcumin as a model drug was encapsulated successfully into FA-SPI nanoparticles, evidenced by X-ray diffraction study. The highest encapsulation and loading efficiencies were around 92.7% and 5.4%, respectively, which were significantly higher (P < 0.05) than those with nonconjugated SPI nanoparticles. In addition, a faster and more complete release of curcumin was observed for FA-SPI nanoparticles in PBS/Tween 20 buffer. Cell culture study showed that conjugation of FA resulted in an increase in cellular uptake by at most 93% in Caco-2 cells. These results suggested that FA-SPI is a potential wall material for encapsulation and enhanced delivery of anticancer drugs.
    • Accession Number:
      0 (Drug Carriers)
      0 (Soybean Proteins)
      935E97BOY8 (Folic Acid)
      IT942ZTH98 (Curcumin)
    • Publication Date:
      Date Created: 20130219 Date Completed: 20140630 Latest Revision: 20191210
    • Publication Date:
      20231215
    • Accession Number:
      10.1021/jf4001567
    • Accession Number:
      23414105