Role of the Vps9-domain protein RgfA in Dictyostelium chemotaxis and development.

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  • Author(s): Hadwiger JA;Hadwiger JA
  • Source:
    Canadian journal of microbiology [Can J Microbiol] 2013 Jan; Vol. 59 (1), pp. 22-7. Date of Electronic Publication: 2012 Nov 06.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Canadian Science Publishing Country of Publication: Canada NLM ID: 0372707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1480-3275 (Electronic) Linking ISSN: 00084166 NLM ISO Abbreviation: Can J Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2011- : Ottawa, ON : Canadian Science Publishing
      Original Publication: Ottawa, Canada : National Research Council, [1954-
    • Subject Terms:
      Chemotaxis/*genetics ; Dictyostelium/*growth & development ; Dictyostelium/*metabolism ; Guanine Nucleotide Exchange Factors/*genetics ; Guanine Nucleotide Exchange Factors/*metabolism; Amino Acid Sequence ; Dictyostelium/genetics ; Endocytosis/genetics ; Folic Acid/metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; Molecular Sequence Data ; Mutation ; Protein Structure, Tertiary ; Receptors, Cell Surface/metabolism ; Sequence Alignment
    • Abstract:
      Proteins with a Vps9 domain function as guanine nucleotide exchange factors for Rab proteins and can mediate the uptake of cell surface receptors or other molecules through endocytosis. However, genes encoding these proteins have not been previously studied in cells with robust chemotactic capabilities. Several genes encoding Vps9 domains were identified in the genome of Dictyostelium discoideum, and one of the genes, designated as rgfA (DDB_G0272038), was examined for functions in cell growth, development, and chemotaxis. The rgfA gene was expressed during vegetative growth and throughout development, but disruption of this gene resulted in no major alterations in cell growth, macropinocytosis, developmental morphology, or chemotactic movement. However, heterologous expression of RgfA resulted in a delay of developmental morphogenesis and impaired chemotaxis of cells to folate but did not affect macropinocytosis. These results suggest that RgfA might share redundant functions with other Dictyostelium Vps9-domain proteins and that heterologous expression of this protein can alter processes that depend on the reception of external signals.
    • Grant Information:
      R15 GM073698-01 United States GM NIGMS NIH HHS; R15 GM097717-01 United States GM NIGMS NIH HHS
    • Accession Number:
      0 (Guanine Nucleotide Exchange Factors)
      0 (Receptors, Cell Surface)
      935E97BOY8 (Folic Acid)
    • Publication Date:
      Date Created: 20130209 Date Completed: 20130408 Latest Revision: 20181202
    • Publication Date:
      20250114
    • Accession Number:
      10.1139/cjm-2012-0519
    • Accession Number:
      23391225