A new approach to determine the stereospecificity in lipase catalysed hydrolysis using circular dichroism (CD): lipases produce optically active diglycerides from achiral triglycerides.

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  • Author(s): Uzawa H;Uzawa H; Nishida Y; Ohrui H; Meguro H
  • Source:
    Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1990 Apr 30; Vol. 168 (2), pp. 506-11.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
    • Publication Information:
      Publication: <2002- >: San Diego, CA : Elsevier
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Diglycerides/*metabolism ; Glycerides/*metabolism ; Lipase/*metabolism ; Triglycerides/*metabolism; Benzyl Alcohols ; Circular Dichroism ; Hydrolysis ; Spectrophotometry, Ultraviolet
    • Abstract:
      We describe a sensitive CD method for determining the stereospecificity in lipase (E.C.3.1.1.3) catalysed hydrolysis of triacyl glycerols into diacyl glycerols. The diglycerols were converted to chiral tert-butyldimethylsilylated 1,2- or 2,3-di-O-benzoyl-sn-glycerol (5 or 5'), and their CD was measured. This approach showed for the first time that lipases produce optically active diacyl glycerides from achiral tripalmitin and tribenzoyl glyceride with a variable extent of enantioselectivity depending on the acyl groups and the enzymes.
    • Accession Number:
      0 (Benzyl Alcohols)
      0 (Diglycerides)
      0 (Glycerides)
      0 (Triglycerides)
      EC 3.1.1.3 (Lipase)
    • Publication Date:
      Date Created: 19900430 Date Completed: 19900604 Latest Revision: 20190612
    • Publication Date:
      20221213
    • Accession Number:
      10.1016/0006-291x(90)92350-9
    • Accession Number:
      2334421