Analysis of strain relatedness using high resolution melting in a case of recurrent candiduria.

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  • Additional Information
    • Source:
      Publisher: BioMed Central Country of Publication: England NLM ID: 100966981 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2180 (Electronic) Linking ISSN: 14712180 NLM ISO Abbreviation: BMC Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: London : BioMed Central, [2001-
    • Subject Terms:
      Candida albicans/*classification ; Candida albicans/*isolation & purification ; Candidiasis/*microbiology ; DNA, Fungal/*genetics ; Molecular Typing/*methods ; Mycological Typing Techniques/*methods; Antifungal Agents/pharmacology ; Candida albicans/drug effects ; Candida albicans/genetics ; Costs and Cost Analysis ; Humans ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Molecular Typing/economics ; Mycological Typing Techniques/economics ; Recurrence ; Transition Temperature
    • Abstract:
      Background: Several genotyping protocols have been described to study Candida albicans strains with different sensitivity values. In this study we have analyzed the genetic relatedness and the antifungal susceptibility of several Candida albicans strains isolated from a patient who from suffered recurrent candiduria for a period of five years. Strains were genotyped using Microsatellite Length Polymorphism (MLP) with three microsatellite markers (HIS 3, EF 3 and CDC 3), and a new method based on high resolution melting (HRM) was developed to analyze the microsatellite region. This method was compared with the conventional technique that uses capillary electrophoresis.
      Results: MICs of the isolates showed the existence of fluconazole susceptible and resistant strains. An inter-colony test using single concentration (8 and 16 mg/l) of fluconazole revealed the coexistence of both fluconazole susceptible and resistant strains. Both genotyping analysis methods showed that all the patient's isolates had a clonal origin. HRM analysis method developed was able to accurately establish strain relatedness and presented a discriminatory power of 0.77.
      Conclusions: Although HRM analysis method presented a lower discriminatory power compared to methods based on capillary electrophoresis, it provided a more cost-effective and suitable alternative for genotyping C. albicans in a clinical laboratory.
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    • Accession Number:
      0 (Antifungal Agents)
      0 (DNA, Fungal)
    • Publication Date:
      Date Created: 20130125 Date Completed: 20130624 Latest Revision: 20231104
    • Publication Date:
      20231215
    • Accession Number:
      PMC3561232
    • Accession Number:
      10.1186/1471-2180-13-13
    • Accession Number:
      23343107