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[Development of immunochromatographic test system for the rapid detection of lipopolysaccharide antigen and cells of causative agent of bovine brucellosis].
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- Additional Information
- Source:
Publisher: Izdatelstvo Nauka Country of Publication: Russia (Federation) NLM ID: 0023416 Publication Model: Print Cited Medium: Print ISSN: 0555-1099 (Print) Linking ISSN: 05551099 NLM ISO Abbreviation: Prikl Biokhim Mikrobiol Subsets: MEDLINE
- Publication Information:
Original Publication: Moskva : Izdatelstvo Nauka
- Subject Terms:
- Abstract:
A rapid method for detection of the surface lipopolysaccharide antigen and the cells of the causative agent of bovine brucellosis was developed. The method represents a sandwich format immunochromatographic assay. The contact between the sample and the test strip with immobilized immunoreagents initiates the fluid movement along the membrane components of the test strip, immunochemical reactions, and the formation of colored bands. The novel method requires 10 minutes to determine the lipopolysaccharide antigen of the cell wall of the brucellosis causative agent at concentrations down to 10 ng/mL and the Brucella abortus cells at concentrations down to 10(6) cells/mL (5 x 10(4) cells in the sample). The specificity of the immunodetection was confirmed. The designed test system can be used for the rapid field diagnosis of brucellosis in cattle.
- Accession Number:
0 (Antibodies, Bacterial)
0 (Antibodies, Immobilized)
0 (Antibodies, Monoclonal)
0 (Antigens, Bacterial)
0 (Lipopolysaccharides)
0 (Reagent Kits, Diagnostic)
0 (Reagent Strips)
- Publication Date:
Date Created: 20130122 Date Completed: 20130205 Latest Revision: 20181202
- Publication Date:
20231215
- Accession Number:
23330393
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