Sequence analysis of hypothetical lysine exporter genes of Rhizobium leguminosarum bv. trifolii from calamine old waste heaps and their evolutionary history.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Oleńska E;Oleńska E; Małek W
  • Source:
    Current microbiology [Curr Microbiol] 2013 May; Vol. 66 (5), pp. 493-8. Date of Electronic Publication: 2013 Jan 16.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Springer International Country of Publication: United States NLM ID: 7808448 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0991 (Electronic) Linking ISSN: 03438651 NLM ISO Abbreviation: Curr Microbiol Subsets: MEDLINE
    • Publication Information:
      Original Publication: New York, Springer International.
    • Subject Terms:
      Amino Acid Transport Systems, Basic/*genetics ; Rhizobium leguminosarum/*genetics; Amino Acid Transport Systems, Basic/chemistry ; Amino Acid Transport Systems, Basic/metabolism ; Bacterial Proteins/genetics ; Base Sequence ; Genotype ; Hazardous Waste ; Lysine/metabolism ; Molecular Sequence Data ; Phylogeny ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Nucleic Acid ; Trifolium/microbiology
    • Abstract:
      The aim of this study was to identify heavy metal detoxification system in Rhizobium leguminosarum bv. trifolii isolated from Trifolium repens inhabiting old (70-100 years) Zn-Pb waste heaps in Poland by PCR reaction with czcD1 and czcD2 primers. By sequence analysis, four different genotypes of obtained amplicons were identified among eight examined isolates. Their sequence similarity ranged 91-99 %. They indicated the highest sequence identity to the hypothetical lysine exporter gene of R. leguminosarum bv. trifolii WSM1325 (91-97 %) and 76-81 % sequence similarity to hypothetical lysine exporter genes of R. leguminosarum bv. trifolii WSM2304 and R. etli CFN42 and CIAT652. On phylogenetic tree of obtained amplicons, all four studied R. leguminosarum bv. trifolii genotypes formed common monophyletic cluster with R. leguminosarum bv. trifolii WSM1325 at 100 % bootstrap support showing that all four amplicons obtained in PCR with czcD1 and czcD2 primers are fragments of hypothetical lysine exporter gene (lysE). We also suggest that Lys efflux exporter may participate in heavy metal transport out of R. leguminosarum bv. trifolii cells.
    • References:
      Stand Genomic Sci. 2010 Jun 15;2(3):347-56. (PMID: 21304718)
      Biotechnol Adv. 2010 May-Jun;28(3):367-74. (PMID: 20149857)
      Proc Natl Acad Sci U S A. 1989 Oct;86(19):7351-5. (PMID: 2678100)
      J Mol Microbiol Biotechnol. 1999 Nov;1(2):327-36. (PMID: 10943564)
      Mol Microbiol. 1994 Mar;11(5):841-7. (PMID: 8022262)
      J Ind Microbiol. 1995 Feb;14(2):61-75. (PMID: 7766212)
      Environ Int. 2007 Apr;33(3):406-13. (PMID: 17275086)
      Toxicol Lett. 2003 Jan 31;137(1-2):65-83. (PMID: 12505433)
      FEMS Microbiol Rev. 2003 Jun;27(2-3):183-95. (PMID: 12829267)
      Ecotoxicol Environ Saf. 2000 Mar;45(3):198-207. (PMID: 10702338)
      Mol Biol Evol. 2007 Aug;24(8):1596-9. (PMID: 17488738)
      J Ind Microbiol. 1995 Feb;14(2):142-53. (PMID: 7766206)
      Nucleic Acids Res. 1997 Dec 15;25(24):4876-82. (PMID: 9396791)
      J Mol Evol. 1980 Dec;16(2):111-20. (PMID: 7463489)
      Chemosphere. 2007 Jun;68(2):360-7. (PMID: 17276484)
      FEMS Microbiol Lett. 1992 Dec 15;100(1-3):197-203. (PMID: 1478456)
      Bioresour Technol. 2012 Jan;104:417-23. (PMID: 22133604)
      FEMS Microbiol Rev. 1994 Jun;14(2):121-37. (PMID: 8049096)
      Trends Biotechnol. 2001 Feb;19(2):67-73. (PMID: 11164556)
      Mol Microbiol. 2002 Feb;43(4):981-91. (PMID: 11936079)
      Eur J Biochem. 1999 Apr;261(1):1-9. (PMID: 10103026)
      FEMS Microbiol Rev. 2003 Jun;27(2-3):239-61. (PMID: 12829270)
      FEMS Microbiol Rev. 2003 Jun;27(2-3):313-39. (PMID: 12829273)
    • Accession Number:
      0 (Amino Acid Transport Systems, Basic)
      0 (Bacterial Proteins)
      0 (Hazardous Waste)
      K3Z4F929H6 (Lysine)
    • Publication Date:
      Date Created: 20130117 Date Completed: 20130913 Latest Revision: 20211021
    • Publication Date:
      20221213
    • Accession Number:
      PMC3611037
    • Accession Number:
      10.1007/s00284-013-0303-z
    • Accession Number:
      23322256