Molecular methods for strain typing of Candida albicans: a review.

Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE

Publication: 2022- : Oxford : Oxford University Press
Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-

Molecular Typing* ; Mycological Typing Techniques*; Candida albicans/*classification; Amplified Fragment Length Polymorphism Analysis ; Candida albicans/genetics ; Microsatellite Repeats ; Multilocus Sequence Typing ; Polymerase Chain Reaction/methods ; Polymorphism, Genetic

Candida albicans is one of the most medically important fungi because of its high frequency as a commensal and pathogenic microorganism causing superficial as well as invasive infections. Strain typing and delineation of the species are essential for understanding its biology, epidemiology and population structure. A wide range of molecular techniques have been used for this purpose including non-DNA-based methods (multi-locus enzyme electrophoresis), conventional DNA-based methods (electrophoretic karyotyping, random amplified polymorphic DNA, amplified fragment length polymorphism, restriction enzyme analysis with and without hybridization, rep-PCR) and DNA-based methods called exact typing methods because they generate unambiguous and highly reproducible typing data (including microsatellite length polymorphism and multi-locus sequence typing). In this review, the main molecular methods used for C. albicans strain typing are summarized, and their advantages and limitations are discussed with regard to their discriminatory power, reproducibility, cost and ease of performance.
(© 2013 The Society for Applied Microbiology.)

Date Created: 20130115 Date Completed: 20140128 Latest Revision: 20181202

20221213

10.1111/jam.12132

23311504