Phorbol esters isolated from Jatropha meal induced apoptosis-mediated inhibition in proliferation of chang and Vero cell lines.

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  • Author(s): Oskoueian E;Oskoueian E; Abdullah N; Ahmad S
  • Source:
    International journal of molecular sciences [Int J Mol Sci] 2012 Oct 24; Vol. 13 (11), pp. 13816-29. Date of Electronic Publication: 2012 Oct 24.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
    • Publication Information:
      Original Publication: Basel, Switzerland : MDPI, [2000-
    • Subject Terms:
      Apoptosis/*drug effects ; Jatropha/*chemistry ; Phorbol Esters/*pharmacology ; Plant Extracts/*pharmacology; Animals ; Caspase 3/metabolism ; Cell Proliferation/drug effects ; Chlorocebus aethiops ; DNA Fragmentation/drug effects ; Dose-Response Relationship, Drug ; Flow Cytometry ; Humans ; Phorbol Esters/isolation & purification ; Plant Extracts/isolation & purification ; Protein Kinase C/metabolism ; Vero Cells
    • Abstract:
      The direct feeding of Jatropha meal containing phorbol esters (PEs) indicated mild to severe toxicity symptoms in various organs of different animals. However, limited information is available on cellular and molecular mechanism of toxicity caused by PEs present in Jatropha meal. Thus, the present study was conducted to determine the cytotoxic and mode of action of PEs isolated from Jatropha meal using human hepatocyte (Chang) and African green monkey kidney (Vero) cell lines. The results showed that isolated PEs inhibited cell proliferation in a dose-dependent manner in both cell lines with the CC(50) of 125.9 and 110.3 μg/mL, respectively. These values were compatible to that of phorbol 12-myristate 13-acetate (PMA) values as positive control i.e., 124.5 and 106.3 μg/mL respectively. Microscopic examination, flow cytometry and DNA fragmentation results confirmed cell death due to apoptosis upon treatment with PEs and PMA at CC(50) concentration for 24 h in both cell lines. The Western blot analysis revealed the overexpression of PKC-δ and activation of caspase-3 proteins which could be involved in the mechanism of action of PEs and PMA. Consequently, the PEs isolated form Jatropha meal caused toxicity and induced apoptosis-mediated proliferation inhibition toward Chang and Vero cell lines involving over-expression of PKC-δ and caspase-3 as their mode of actions.
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    • Contributed Indexing:
      Note: Original DateCompleted: 20121204
    • Accession Number:
      0 (Phorbol Esters)
      0 (Plant Extracts)
      EC 2.7.11.13 (Protein Kinase C)
      EC 3.4.22.- (Caspase 3)
    • Publication Date:
      Date Created: 20121204 Date Completed: 20150423 Latest Revision: 20211021
    • Publication Date:
      20231215
    • Accession Number:
      PMC3509552
    • Accession Number:
      10.3390/ijms131113816
    • Accession Number:
      23203036