Gbx2 plays an essential but transient role in the formation of thalamic nuclei.

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  • Author(s): Li K;Li K; Zhang J; Li JY
  • Source:
    PloS one [PLoS One] 2012; Vol. 7 (10), pp. e47111. Date of Electronic Publication: 2012 Oct 04.
  • Publication Type:
    Journal Article; Research Support, N.I.H., Extramural
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
    • Publication Information:
      Original Publication: San Francisco, CA : Public Library of Science
    • Subject Terms:
      Homeodomain Proteins/*metabolism ; Neurons/*cytology ; Neurons/*metabolism ; Thalamic Nuclei/*cytology ; Thalamic Nuclei/*metabolism; Animals ; Cell Survival/genetics ; Cell Survival/physiology ; Cerebral Cortex/cytology ; Cerebral Cortex/metabolism ; Female ; Fluorescent Antibody Technique ; Gene Expression Regulation, Developmental/genetics ; Gene Expression Regulation, Developmental/physiology ; Homeodomain Proteins/genetics ; In Situ Hybridization ; Male ; Mice
    • Abstract:
      Unlike the laminar arrangement of neurons in the neocortex, thalamic neurons aggregate to form about dozens of nuclei, many of which make topographic connections with specific areas in the neocortex. The molecular mechanisms underlying the formation of thalamic nuclei remain largely unknown. Homeodomain transcription factor Gbx2 is specifically expressed in the developing thalamus. Deleting Gbx2 leads to severe disruption of the histogenesis of the thalamus in mice, demonstrating an essential role of Gbx2 in this brain structure. Using inducible genetic fate mapping, we have previously shown that the neuronal precursors for different sets of thalamic nuclei have distinctive onset and duration of Gbx2 expression, suggesting that the dynamic expression of Gbx2 plays an important role in the specification and differentiation of thalamic nuclei. Here, we showed that the Gbx2 lineage exclusively gives rise to neurons but not glia in the thalamus. We performed conditional deletion to examine the temporal requirements of Gbx2 in the developing thalamus in mice. Corresponding to the dynamic and differential expression of Gbx2 in various thalamic nucleus groups, deleting Gbx2 at different embryonic stages disrupts formation of distinct sets of thalamic nuclei. Interestingly, different thalamic nuclei have remarkably different requirements of Gbx2 for the survival of thalamic neurons. Furthermore, although Gbx2 expression persists in many thalamic nuclei until adulthood, only the initial expression of Gbx2 following neurogenesis is crucial for the differentiation of thalamic nuclei. Our results indicate that the dynamic expression of Gbx2 may act as an important determinant in coupling with other developmental programs to generate distinct thalamic nuclei.
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    • Grant Information:
      R01 MH094914 United States MH NIMH NIH HHS
    • Accession Number:
      0 (Gbx2 protein, mouse)
      0 (Homeodomain Proteins)
    • Publication Date:
      Date Created: 20121012 Date Completed: 20130531 Latest Revision: 20211021
    • Publication Date:
      20221213
    • Accession Number:
      PMC3464241
    • Accession Number:
      10.1371/journal.pone.0047111
    • Accession Number:
      23056596