Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
Hyperosmolarity-induced lipid droplet formation depends on ceramide production by neutral sphingomyelinase 2.
Item request has been placed!
×
Item request cannot be made.
×
![loading](/sites/all/modules/hf_eds/images/loading.gif)
Processing Request
- Author(s): Robciuc A;Robciuc A; Hyötyläinen T; Jauhiainen M; Holopainen JM
- Source:
Journal of lipid research [J Lipid Res] 2012 Nov; Vol. 53 (11), pp. 2286-95. Date of Electronic Publication: 2012 Aug 15.
- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
- Language:
English
- Additional Information
- Source:
Publisher: Elsevier Country of Publication: United States NLM ID: 0376606 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1539-7262 (Electronic) Linking ISSN: 00222275 NLM ISO Abbreviation: J Lipid Res Subsets: MEDLINE
- Publication Information:
Publication: 2021- : [New York] : Elsevier
Original Publication: Memphis, Lipid Research, inc.
- Subject Terms:
- Abstract:
Hyperosmolarity (HO) imposes a remarkable stress on membranes, especially in tissues in direct contact with the external environment. Our efforts were focused on revealing stress-induced lipid changes that precede the inflammatory cytokine response in human corneal epithelial cells exposed to increasing osmolarity. We used a lipidomic analysis that detected significant and systematic changes in the lipid profile, highly correlated with sodium concentrations in the medium. Ceramides and triglycerides (TGs) were the most-responsive lipid classes, with gradual increases of up to 2- and 3-fold, respectively, when compared with control. The source of ceramide proved to be sphingomyelin hydrolysis, and neutral sphingomyelinase 2 (NSM2) activity showed a 2-fold increase 1 h after HO stress, whereas transcription increased 3-fold. Both TG accumulation and IL-8 secretion were shown to be dependent on ceramide production by specific knock-down of NSM2. In HCE cells, diglyceride acyltransferase 1 was responsible for the TG synthesis, but the enzyme activity had no effect on cytokine secretion. Hence, NSM2 plays a key role in the cellular response to hyperosmolar stress, and its activity regulates both cytokine secretion and lipid droplet formation.
- References:
Nature. 1996 Jun 27;381(6585):800-3. (PMID: 8657285)
J Biol Chem. 2010 Feb 26;285(9):6693-705. (PMID: 20032468)
Ocul Surf. 2007 Apr;5(2):75-92. (PMID: 17508116)
J Lipid Res. 2012 Jul;53(7):1245-53. (PMID: 22534641)
Methods Enzymol. 2007;434:257-64. (PMID: 17954252)
J Biol Chem. 2010 Apr 2;285(14):10213-22. (PMID: 20106976)
Ocul Surf. 2007 Apr;5(2):93-107. (PMID: 17508117)
Invest Ophthalmol Vis Sci. 2006 Oct;47(10):4309-15. (PMID: 17003420)
Methods Mol Biol. 2011;708:247-57. (PMID: 21207295)
J Biol Chem. 2008 Oct 10;283(41):27369-27382. (PMID: 18632668)
Biochim Biophys Acta. 2007 Jan;1771(1):31-44. (PMID: 17150408)
Cell. 1997 Nov 28;91(5):605-15. (PMID: 9393854)
J Lipid Res. 2012 Sep;53(9):1800-10. (PMID: 22701043)
J Biol Chem. 2007 Jan 12;282(2):1384-96. (PMID: 17085432)
J Biol Chem. 2009 Jan 23;284(4):2383-96. (PMID: 19036730)
Can J Biochem Physiol. 1959 Aug;37(8):911-7. (PMID: 13671378)
J Biol Chem. 2009 Feb 27;284(9):5697-708. (PMID: 19117952)
Toxicol In Vitro. 2002 Aug;16(4):349-55. (PMID: 12110272)
Neurochem Int. 2002 Apr;40(4):327-36. (PMID: 11792463)
Science. 1994 Aug 5;265(5173):808-11. (PMID: 7914033)
Mol Cancer. 2010 Sep 13;9:239. (PMID: 20836852)
J Lipid Res. 2011 Nov;52(11):2012-20. (PMID: 21900174)
Nat Immunol. 2007 Dec;8(12):1295-301. (PMID: 18026082)
J Cell Physiol. 1996 Apr;167(1):60-71. (PMID: 8698841)
Biophys J. 2006 Jun 1;90(11):4085-92. (PMID: 16533839)
J Inflamm (Lond). 2009 Jun 23;6:21. (PMID: 19549308)
Invest Ophthalmol Vis Sci. 1995 Mar;36(3):614-21. (PMID: 7534282)
Cornea. 2009 Oct;28(9):1023-7. (PMID: 19724208)
Am J Pathol. 2002 Sep;161(3):987-96. (PMID: 12213727)
Cornea. 1998 Jan;17(1):38-56. (PMID: 9436879)
Proc Natl Acad Sci U S A. 1996 Oct 1;93(20):11091-6. (PMID: 8855314)
Int Arch Allergy Immunol. 1993;101(2):136-42. (PMID: 8508051)
Methods Enzymol. 1986;129:716-38. (PMID: 3724551)
Prog Lipid Res. 2001 Sep;40(5):325-438. (PMID: 11470496)
Arch Ophthalmol. 2003 Jun;121(6):825-31. (PMID: 12796254)
BMC Bioinformatics. 2010 Jul 23;11:395. (PMID: 20650010)
Biochim Biophys Acta. 2003 Oct 20;1634(1-2):1-14. (PMID: 14563408)
- Accession Number:
0 (Interleukin-8)
0 (Sphingomyelins)
EC 3.1.4.12 (Sphingomyelin Phosphodiesterase)
- Publication Date:
Date Created: 20120818 Date Completed: 20130306 Latest Revision: 20211021
- Publication Date:
20221213
- Accession Number:
PMC3465998
- Accession Number:
10.1194/jlr.M026732
- Accession Number:
22899568
No Comments.