Evaluation of the Standard Diagnostics Leptospira IgM ELISA for diagnosis of acute leptospirosis in Lao PDR.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 7506129 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3503 (Electronic) Linking ISSN: 00359203 NLM ISO Abbreviation: Trans R Soc Trop Med Hyg Subsets: MEDLINE
    • Publication Information:
      Publication: 2013- : Oxford : Oxford University Press
      Original Publication: 1920- : London : Royal Society of Tropical Medicine and Hygiene
    • Subject Terms:
      Agglutination Tests* ; Enzyme-Linked Immunosorbent Assay*; Fever/*microbiology ; Leptospira/*isolation & purification ; Leptospirosis/*diagnosis; Antibodies, Bacterial ; Fever/epidemiology ; Humans ; Immunoglobulin M ; Laos/epidemiology ; Leptospira/immunology ; Leptospirosis/epidemiology ; Reproducibility of Results ; Sensitivity and Specificity
    • Abstract:
      The diagnostic utility of the Standard Diagnostics Leptospira IgM ELISA for detection of acute leptospirosis was assessed in febrile adults admitted in Vientiane, Laos. Using the cut-off suggested by the manufacturer [optical density (OD) ≥0.75], the assay demonstrated limited diagnostic capacity with a sensitivity of 95% and a specificity of 41% compared with the Leptospira microscopic agglutination test, which is the serological gold standard. However, re-evaluation of the diagnostic cut-off to an OD of 1.7 demonstrated improved diagnostic accuracy overall (sensitivity 70%; specificity 78%).
      (Copyright © 2012 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.)
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    • Grant Information:
      United Kingdom WT_ Wellcome Trust; 089275 United Kingdom WT_ Wellcome Trust
    • Accession Number:
      0 (Antibodies, Bacterial)
      0 (Immunoglobulin M)
    • Publication Date:
      Date Created: 20120724 Date Completed: 20121218 Latest Revision: 20220330
    • Publication Date:
      20250114
    • Accession Number:
      PMC3464426
    • Accession Number:
      10.1016/j.trstmh.2012.06.002
    • Accession Number:
      22818757