Changes in adhesion ability of Aeromonas hydrophila during long exposure to salt stress conditions.

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  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2022- : Oxford : Oxford University Press
      Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-
    • Subject Terms:
      Bacterial Adhesion*; Aeromonas hydrophila/*physiology ; Sodium Chloride/*pharmacology; Aeromonas hydrophila/growth & development ; Aeromonas hydrophila/pathogenicity ; Cell Line ; Humans ; Microscopy, Electron ; Osmolar Concentration ; Temperature
    • Abstract:
      Aims: Stressful environmental conditions influence both bacterial growth and expression of virulence factors. In the present study, we evaluated the influence of NaCl on Aeromonas hydrophila adhesiveness at two temperatures. This agent is often involved in clinical cases; however, its pathogenic potential is still not fully understood.
      Methods and Results: Bacteria were grown in presence of 1·7%, 3·4%, 6·0% NaCl over a 188 day period and then reinoculated in fresh Nutrient Broth with incubation at 4 and 24°C. Bacterial adhesiveness was tested on Hep-2 cells, and specimens were processed for light, scanning and transmission electron microscopy. Adhesive capacity decreased over time with an increase in reduction percentages depending on NaCl concentrations. At 1·7% NaCl, the reduction was apparently temporary and adhesiveness rapidly recovered in revitalized bacteria, while 3·4%, 6·0% NaCl seemed to be detrimental. Normal, elongated and filamentous bacteria retained adhesiveness capability, although with reduced expression, while in spherical cells, this property seemed to be lost or dramatically reduced.
      Conclusions: Our study shows that high osmolarity plays a significant role in adhesion inhibition, therefore having possible implications in the pathogenesis of the infections by Aer. hydrophila.
      Significance and Impact of the Study: This study intends to give a contribution to a better understanding of the pathogenic role of this bacterium whose pathogenicity is still under debate.
      (© 2012 The Authors Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.)
    • Accession Number:
      451W47IQ8X (Sodium Chloride)
    • Publication Date:
      Date Created: 20120719 Date Completed: 20130617 Latest Revision: 20181201
    • Publication Date:
      20231215
    • Accession Number:
      10.1111/j.1365-2672.2012.05399.x
    • Accession Number:
      22805151