Development of a fluorescence immunochromatographic assay for the detection of zeta globin in the blood of (--(SEA)) α-thalassemia carriers.

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  • Author(s): Wen L;Wen L; Zhu P; Liu Y; Pan Q; Qu Y; Xu X; Li X; Fu N
  • Source:
    Blood cells, molecules & diseases [Blood Cells Mol Dis] 2012 Oct 15-Dec 15; Vol. 49 (3-4), pp. 128-32. Date of Electronic Publication: 2012 Jun 05.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 9509932 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0961 (Electronic) Linking ISSN: 10799796 NLM ISO Abbreviation: Blood Cells Mol Dis Subsets: MEDLINE
    • Publication Information:
      Publication: <1996-> : Orlando, FL : Academic Press
      Original Publication: La Jolla, Calif. : Blood Cells Foundation, 1995-
    • Subject Terms:
      Chromatography, Affinity*; alpha-Thalassemia/*diagnosis ; alpha-Thalassemia/*genetics ; zeta-Globins/*analysis; Adult ; Antibodies, Monoclonal ; Asia, Southeastern ; Base Sequence ; Case-Control Studies ; Enzyme-Linked Immunosorbent Assay ; Fluorescence ; Genetic Testing ; Heterozygote ; Homozygote ; Humans ; Molecular Sequence Data ; Polymerase Chain Reaction ; Sensitivity and Specificity ; Sequence Deletion ; alpha-Thalassemia/blood ; zeta-Globins/genetics
    • Abstract:
      Southeast Asian deletion (--(SEA)) α-thalassemia is an inherited monogenic disorder of human hemoglobin, and embryonic globin ζ (hemoglobin ζ, zeta globin chain or Hb zeta chain) has been shown to be a marker that can be used for the identification of carriers of the (--(SEA)) α-thalassemia deletion. In this work, a fluorescence immunochromatographic assay (FL-ICA) was established to detect the zeta globin chain in the hemolysates of carriers of the (--(SEA)) α-thalassemia deletion. This assay can be completed within 10min using a simple UV detector and does not suffer from interference from the red background color of the hemolysate. A total of 314 blood samples were tested by FL-ICA and ELISA. The results of these assays were confirmed by PCR, the standard technique for genetic disease testing. The sensitivity and specificity of this novel FL-ICA were 100% and 98.0%, respectively; the corresponding values for the ELISA performed simultaneously were 100% and 99.2%, respectively. In conclusion, a new FL-ICA-a simple, fast, convenient, low-cost method-was developed that may be useful in both high-throughput screening and individual detection of the (--(SEA)) α-thalassemia deletion in carriers. Additionally, this qualitative FL-ICA may enlighten the development of a new systems for analysis of other target molecules using whole-blood samples.
      (Copyright © 2012 Elsevier Inc. All rights reserved.)
    • Accession Number:
      0 (Antibodies, Monoclonal)
      0 (zeta-Globins)
    • Publication Date:
      Date Created: 20120609 Date Completed: 20130308 Latest Revision: 20220409
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.bcmd.2012.05.011
    • Accession Number:
      22677106