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Overfeeding reduces insulin sensitivity and increases oxidative stress, without altering markers of mitochondrial content and function in humans.
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- Author(s): Samocha-Bonet D;Samocha-Bonet D; Campbell LV; Mori TA; Croft KD; Greenfield JR; Turner N; Heilbronn LK
- Source:
PloS one [PLoS One] 2012; Vol. 7 (5), pp. e36320. Date of Electronic Publication: 2012 May 07.- Publication Type:
Journal Article; Research Support, Non-U.S. Gov't- Language:
English - Source:
- Additional Information
- Source: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
- Publication Information: Original Publication: San Francisco, CA : Public Library of Science
- Subject Terms: Diabetes Mellitus, Type 2*/metabolism ; Diabetes Mellitus, Type 2*/physiopathology ; Insulin*/blood ; Insulin Resistance* ; Obesity*/metabolism ; Obesity*/pathology ; Oxidative Stress*; Adult ; Biopsy ; Blood Glucose ; Body Weight ; Citrate (si)-Synthase/metabolism ; Cohort Studies ; Diet, High-Fat ; Female ; Humans ; Intracellular Signaling Peptides and Proteins/metabolism ; Lipid Metabolism ; Male ; Mitochondria/metabolism ; Mitochondria/physiology ; Muscle, Skeletal/enzymology ; Muscle, Skeletal/metabolism ; Weight Gain/physiology
- Abstract: Background: Mitochondrial dysfunction and increased oxidative stress are associated with obesity and type 2 diabetes. High fat feeding induces insulin resistance and increases skeletal muscle oxidative stress in rodents, but there is controversy as to whether skeletal muscle mitochondrial biogenesis and function is altered.
Methodology and Principal Findings: Forty (37 ± 2 y) non-obese (25.6 ± 0.6 kg/m(2)) sedentary men (n = 20) and women (n = 20) were overfed (+1040 ± 100 kcal/day, 46 ± 1% of energy from fat) for 28 days. Hyperinsulinemic-euglycemic clamps were performed at baseline and day 28 of overfeeding and skeletal muscle biopsies taken at baseline, day 3 and day 28 of overfeeding in a sub cohort of 26 individuals (13 men and 13 women) that consented to having all 3 biopsies performed. Weight increased on average in the whole cohort by 0.6 ± 0.1 and 2.7 ± 0.3 kg at days 3 and 28, respectively (P<0.0001, without a significant difference in the response between men and women (P = 0.4). Glucose infusion rate during the hyperinsulinemic-euglycemic clamp decreased from 54.8 ± 2.8 at baseline to 50.3 ± 2.5 µmol/min/kg FFM at day 28 of overfeeding (P = 0.03) without a significant difference between men and women (P = 0.4). Skeletal muscle protein carbonyls and urinary F2-isoprostanes increased with overfeeding (P<0.05). Protein levels of muscle peroxisome proliferator-activated receptor gamma coactivator-1α (PGC1α) and subunits from complex I, II and V of the electron transport chain were increased at day 3 (all P<0.05) and returned to basal levels at day 28. No changes were detected in muscle citrate synthase activity or ex vivo CO(2) production at either time point.
Conclusions: Peripheral insulin resistance was induced by overfeeding, without reducing any of the markers of mitochondrial content that were examined. Oxidative stress was however increased, and may have contributed to the reduction in insulin sensitivity observed. - References: Diabetologia. 2010 Aug;53(8):1700-8. (PMID: 20461357)
Diabetes. 2010 Oct;59(10):2444-52. (PMID: 20682693)
J Appl Physiol Respir Environ Exerc Physiol. 1984 Jan;56(1):78-83. (PMID: 6693336)
Pflugers Arch. 2002 Dec;445(3):431-6. (PMID: 12466947)
Trends Endocrinol Metab. 2010 Feb;21(2):83-8. (PMID: 19854062)
FASEB J. 2004 Dec;18(15):1791-800. (PMID: 15576482)
Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7815-20. (PMID: 18509063)
Mol Cell Proteomics. 2007 Apr;6(4):624-37. (PMID: 17205980)
Int J Obes Relat Metab Disord. 2002 Jan;26(1):65-72. (PMID: 11791148)
Eur J Endocrinol. 2008 May;158(5):643-53. (PMID: 18426822)
J Clin Endocrinol Metab. 2011 Mar;96(3):775-81. (PMID: 21190973)
J Physiol. 2009 May 15;587(Pt 10):2387-97. (PMID: 19332493)
J Biol Chem. 2005 Mar 18;280(11):10290-7. (PMID: 15598661)
Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17787-92. (PMID: 19805130)
J Clin Endocrinol Metab. 2007 Apr;92(4):1467-73. (PMID: 17244782)
FEBS Lett. 2008 Feb 20;582(4):510-6. (PMID: 18230360)
Diabetologia. 2006 Oct;49(10):2419-26. (PMID: 16896940)
Am J Physiol Endocrinol Metab. 2010 Jul;299(1):E33-8. (PMID: 20442322)
Diabetes. 2001 Nov;50(11):2579-84. (PMID: 11679437)
Trends Endocrinol Metab. 2008 Nov;19(9):324-30. (PMID: 18804383)
J Nutr Metab. 2012;2012:238056. (PMID: 22474579)
Diabetes Care. 2007 Oct;30(10):2529-35. (PMID: 17586736)
J Proteome Res. 2010 May 7;9(5):2226-35. (PMID: 20143783)
Cell Metab. 2009 Oct;10(4):260-72. (PMID: 19808019)
Nature. 2006 Apr 13;440(7086):944-8. (PMID: 16612386)
Diabetes. 1991 Nov;40(11):1397-403. (PMID: 1936601)
Diabetes. 2010 Sep;59(9):2164-70. (PMID: 20547978)
J Clin Endocrinol Metab. 2010 Jun;95(6):3048-56. (PMID: 20410232)
J Clin Invest. 2009 Mar;119(3):573-81. (PMID: 19188683)
Diabetes. 2008 May;57(5):1166-75. (PMID: 18285554)
Diabetes. 2005 Jul;54(7):1926-33. (PMID: 15983191)
J Biol Chem. 2005 Sep 30;280(39):33588-98. (PMID: 16079133)
Anal Biochem. 1999 Mar 1;268(1):117-25. (PMID: 10036170)
Diabetes. 2007 Aug;56(8):2085-92. (PMID: 17519422) - Molecular Sequence: ClinicalTrials.gov NCT00562393
- Accession Number: 0 (Blood Glucose)
0 (Insulin)
0 (Intracellular Signaling Peptides and Proteins)
0 (src kinase associated phosphoprotein 2)
EC 2.3.3.1 (Citrate (si)-Synthase) - Publication Date: Date Created: 20120516 Date Completed: 20120926 Latest Revision: 20211021
- Publication Date: 20240829
- Accession Number: PMC3346759
- Accession Number: 10.1371/journal.pone.0036320
- Accession Number: 22586466
- Source:
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