ROS production during symbiotic infection suppresses pathogenesis-related gene expression.

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  • Additional Information
    • Source:
      Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101291431 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1559-2324 (Electronic) Linking ISSN: 15592316 NLM ISO Abbreviation: Plant Signal Behav Subsets: MEDLINE
    • Publication Information:
      Publication: 2015- : Philadelphia, PA : Taylor & Francis
      Original Publication: Georgetown, TX : Landes Bioscience
    • Subject Terms:
      Medicago truncatula/*metabolism ; Medicago truncatula/*microbiology ; Reactive Oxygen Species/*metabolism ; Sinorhizobium meliloti/*physiology ; Symbiosis/*physiology; Cytosol/metabolism ; Gene Expression Regulation, Plant/genetics ; Gene Expression Regulation, Plant/physiology ; Hydrogen Peroxide/pharmacology ; Medicago truncatula/genetics ; Pseudomonas putida/metabolism ; Pseudomonas putida/pathogenicity ; Sinorhizobium meliloti/genetics ; Sodium Chloride/pharmacology ; Symbiosis/genetics ; Vacuoles/metabolism
    • Abstract:
      Leguminous plants have exclusive ability to form symbiotic relationship with soil bacteria of the genus Rhizobium. Symbiosis is a complex process that involves multiple molecular signaling activities, such as calcium fluxes, production of reactive oxygen species (ROS) and synthesis of nodulation genes. We analyzed the role of ROS in defense gene expression in Medicago truncatula during symbiosis and pathogenesis. Studies in Arabidopsis thaliana showed that the induction of pathogenesis-related (PR) genes during systemic acquired resistance (SAR) is regulated by NPR1 protein, which resides in the cytoplasm as an oligomer. After oxidative burst and return of reducing conditions, the NPR1 undergoes monomerization and becomes translocated to the nucleus, where it functions in PR genes induction. We show that ROS production is both stronger and longer during symbiotic interactions than during interactions with pathogenic, nonhost or common nonpathogenic soil bacteria. Moreover, root cells inoculated with Sinorhizobium meliloti accumulated ROS in the cytosol but not in vacuoles, as opposed to Pseudomonas putida inoculation or salt stress treatment. Furthermore, increased ROS accumulation by addition of H₂O₂ reduced the PR gene expression, while catalase had an opposite effect, establishing that the PR gene expression is opposite to the level of cytoplasmic ROS. In addition, we show that salicylic acid pretreatment significantly reduced ROS production in root cells during symbiotic interaction.
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    • Accession Number:
      0 (Reactive Oxygen Species)
      451W47IQ8X (Sodium Chloride)
      BBX060AN9V (Hydrogen Peroxide)
    • Publication Date:
      Date Created: 20120414 Date Completed: 20130128 Latest Revision: 20240321
    • Publication Date:
      20240321
    • Accession Number:
      PMC3443923
    • Accession Number:
      10.4161/psb.19217
    • Accession Number:
      22499208