Inhibitory effects of silver ions on Legionella pneumophila grown on agar, intracellular in Acanthamoeba castellanii and in artificial biofilms.

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  • Author(s): Unger C;Unger C; Lück C
  • Source:
    Journal of applied microbiology [J Appl Microbiol] 2012 Jun; Vol. 112 (6), pp. 1212-9. Date of Electronic Publication: 2012 Apr 10.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9706280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-2672 (Electronic) Linking ISSN: 13645072 NLM ISO Abbreviation: J Appl Microbiol Subsets: MEDLINE
    • Publication Information:
      Publication: 2022- : Oxford : Oxford University Press
      Original Publication: Oxford : Published for the Society for Applied Bacteriology by Blackwell Science, c1997-
    • Subject Terms:
      Biofilms*; Acanthamoeba castellanii/*microbiology ; Legionella pneumophila/*drug effects ; Legionella pneumophila/*growth & development ; Silver Nitrate/*pharmacology; Agar ; Bacteriological Techniques
    • Abstract:
      Aims: We undertook a series of experiments to investigate the susceptibility of Legionella pneumophila grown under extracellular and intracellular conditions and other water-related bacteria to silver ions.
      Methods and Results: In this study, the antimicrobial effect of silver ions to intra- and extra-cellular grown Legionella bacteria was investigated. The minimal inhibitory concentration (MIC) after 24 h exposure, leading to a 5 log reduction, was c. 64 μg l(-1) AgNO(3) for extracellular grown Legionella and other tested Gram-positive and Gram-negative bacteria. In contrast, the MIC for intracellularly grown Legionella was up to 4096 μg l(-1) AgNO(3) after 24 h. Furthermore, the heterotrophic bacteria grown within a biofilm model were killed at a concentration of 4-16 μg l(-1) AgNO(3). In contrast, biofilm-associated Legionella were less sensitive (MIC 128-512 μg l(-1) AgNO(3)).
      Conclusion: Intracellularly and biofilm-grown legionellae are less sensitive against silver compared with agar-grown bacteria.
      Significance and Impact of the Study: The reduced sensitivity of Legionella grown in amoebae might explain why the effect of silver decontamination requires an extended exposure in field trials.
      (© 2012 The Authors. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.)
    • Accession Number:
      9002-18-0 (Agar)
      95IT3W8JZE (Silver Nitrate)
    • Publication Date:
      Date Created: 20120315 Date Completed: 20121126 Latest Revision: 20181201
    • Publication Date:
      20240829
    • Accession Number:
      10.1111/j.1365-2672.2012.05285.x
    • Accession Number:
      22413947