Overexpression of the AtLOS5 gene increased abscisic acid level and drought tolerance in transgenic cotton.

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  • Author(s): Yue Y;Yue Y; Zhang M; Zhang J; Tian X; Duan L; Li Z
  • Source:
    Journal of experimental botany [J Exp Bot] 2012 Jun; Vol. 63 (10), pp. 3741-8. Date of Electronic Publication: 2012 Mar 12.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 9882906 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1460-2431 (Electronic) Linking ISSN: 00220957 NLM ISO Abbreviation: J Exp Bot Subsets: MEDLINE
    • Publication Information:
      Original Publication: Oxford, UK : Oxford University Press,
    • Subject Terms:
      Gene Expression*; Abscisic Acid/*biosynthesis ; Arabidopsis Proteins/*genetics ; Arabidopsis Proteins/*metabolism ; Gossypium/*physiology ; Plants, Genetically Modified/*physiology ; Sulfurtransferases/*genetics ; Sulfurtransferases/*metabolism; Droughts ; Gene Expression Regulation, Plant ; Gossypium/genetics ; Plants, Genetically Modified/genetics ; Stress, Physiological ; Water/metabolism
    • Abstract:
      Drought is the major environmental stress that limits cotton (Gossypium hirsutum L.) production worldwide. LOS5/ABA3 (LOS5) encodes a molybdenum co-factor and is essential for activating aldehyde oxidase, which is involved in abscisic acid (ABA) biosynthesis. In this study, a LOS5 cDNA of Arabidopsis thaliana was overexpressed in cotton cultivar Zhongmiansuo35 (Z35) by Agrobacterium tumefaciens-mediated transformation. The transformation and overexpression of AtLOS5 were assessed by PCR and RT-PCR analysis. Detached shoots of transgenic cotton showed slower transpirational water loss than those of Z35. When pot-grown 6-week-old seedlings were withheld from watering for 3 d, transgenic cotton accumulated 25% more endogenous ABA and about 20% more proline than Z35 plants. The transgenic plants also showed increased expression of some drought-responding genes such as P5CS and RD22, and enhanced activity of antioxidant enzymes such as superoxide dismutase, peroxidase, and ascorbate peroxidase. Their membrane integrity was considerably improved under water stress, as indicated by reduced malondialdehyde content and electrolyte leakage relative to control plants. When the pot-grown plants were subjected to deficit irrigation for 8 weeks (watering to 50% of field capacity), transgenic plants showed a 13% increase in fresh weight than the wild type under the same drought condition. These results suggest that the AtLOS5 transgenic cotton plants acquired a better drought tolerance through enhanced ABA production and ABA-induced physiological regulations.
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    • Accession Number:
      0 (Arabidopsis Proteins)
      059QF0KO0R (Water)
      72S9A8J5GW (Abscisic Acid)
      EC 2.8.1.- (ABA3 protein, Arabidopsis)
      EC 2.8.1.- (Sulfurtransferases)
    • Publication Date:
      Date Created: 20120314 Date Completed: 20121123 Latest Revision: 20211021
    • Publication Date:
      20240829
    • Accession Number:
      PMC3388821
    • Accession Number:
      10.1093/jxb/ers069
    • Accession Number:
      22412184