Multiple cis-regulatory elements are involved in the complex regulation of the sieve element-specific MtSEO-F1 promoter from Medicago truncatula.

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  • Additional Information
    • Source:
      Publisher: Wiley Country of Publication: England NLM ID: 101148926 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1438-8677 (Electronic) Linking ISSN: 14358603 NLM ISO Abbreviation: Plant Biol (Stuttg) Subsets: MEDLINE
    • Publication Information:
      Publication: Oxford, England : Wiley
      Original Publication: Stuttgart : New York, NY : G. Thieme Verlag ; Thieme New York, c1999-
    • Subject Terms:
      Promoter Regions, Genetic*; Medicago truncatula/*genetics ; Phloem/*genetics ; Plant Proteins/*genetics; Gene Expression Regulation, Plant ; Mutagenesis, Site-Directed ; Organ Specificity/genetics ; Phloem/cytology ; Plant Proteins/metabolism ; Sequence Deletion ; TATA Box/genetics ; Transcription Initiation Site
    • Abstract:
      The sieve element occlusion (SEO) gene family includes several members that are expressed specifically in immature sieve elements (SEs) in the developing phloem of dicotyledonous plants. To determine how this restricted expression profile is achieved, we analysed the SE-specific Medicago truncatula SEO-F1 promoter (PMtSEO-F1) by constructing deletion, substitution and hybrid constructs and testing them in transgenic tobacco plants using green fluorescent protein as a reporter. This revealed four promoter regions, each containing cis-regulatory elements that activate transcription in SEs. One of these segments also contained sufficient information to suppress PMtSEO-F1 transcription in the phloem companion cells (CCs). Subsequent in silico analysis revealed several candidate cis-regulatory elements that PMtSEO-F1 shares with other SEO promoters. These putative sieve element boxes (PSE boxes) are promising candidates for cis-regulatory elements controlling the SE-specific expression of PMtSEO-F1.
      (© 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.)
    • Contributed Indexing:
      Keywords: Companion cell; forisome; immature sieve element; phloem; promoter; reporter gene; sieve element occlusion
    • Accession Number:
      0 (Plant Proteins)
    • Publication Date:
      Date Created: 20120313 Date Completed: 20160530 Latest Revision: 20150824
    • Publication Date:
      20221213
    • Accession Number:
      10.1111/j.1438-8677.2011.00556.x
    • Accession Number:
      22404711