Analytical approaches to detection and characterization of disease-linked chromosome aberrations.

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  • Additional Information
    • Source:
      Publisher: Nature Publishing Group Country of Publication: England NLM ID: 8702459 Publication Model: Print Cited Medium: Print ISSN: 0268-3369 (Print) Linking ISSN: 02683369 NLM ISO Abbreviation: Bone Marrow Transplant Subsets: MEDLINE
    • Publication Information:
      Publication: <2003->: London : Nature Publishing Group
      Original Publication: Basingstoke, Hampshire : Scientific & Medical Division, Macmillan Press, c1986-
    • Subject Terms:
      Chromosome Aberrations*; Cytogenetics/*methods ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics; DNA, Neoplasm/genetics ; Fluorescent Dyes ; Humans ; Karyotyping/methods ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis ; Molecular Probes ; Neoplasms/diagnosis ; Neoplasms/genetics ; Nucleic Acid Hybridization
    • Abstract:
      Flow karyotyping and FISH with chromosome specific or disease-locus-specific probes are powerful adjuncts to conventional cytogenetic analysis. Flow karyotyping is well suited to quantitative analysis of DNA content changes that occur during structural rearrangement. FISH with probes for repeated sequences allows ready detection of aneuploidy in interphase cells. FISH with whole chromosome composite probes to metaphase spreads facilitates detection of subtle structural changes and allows detection of structural aberrations that occur at frequencies as low as 10(-3). FISH with locus-specific probes facilitates diagnosis of specific genetic diseases, allows phenotype-genotype correlation on a cell-by-cell basis and may be developed into a sensitive method for detection of residual disease.
    • Number of References:
      23
    • Accession Number:
      0 (DNA, Neoplasm)
      0 (Fluorescent Dyes)
      0 (Molecular Probes)
    • Publication Date:
      Date Created: 19900701 Date Completed: 19901004 Latest Revision: 20071115
    • Publication Date:
      20240829
    • Accession Number:
      2202466