Isolation of a yeast gene, SRH1, that encodes a homologue of the 54K subunit of mammalian signal recognition particle.

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  • Author(s): Amaya Y;Amaya Y; Nakano A; Ito K; Mori M
  • Source:
    Journal of biochemistry [J Biochem] 1990 Mar; Vol. 107 (3), pp. 457-63.
  • Publication Type:
    Journal Article
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Oxford University Press Country of Publication: England NLM ID: 0376600 Publication Model: Print Cited Medium: Print ISSN: 0021-924X (Print) Linking ISSN: 0021924X NLM ISO Abbreviation: J Biochem Subsets: MEDLINE
    • Publication Information:
      Publication: : Abingdon, UK : Oxford University Press
      Original Publication: Tokyo : Japanese Biochemical Society
    • Subject Terms:
      Fungal Proteins* ; Genes, Fungal* ; Saccharomyces cerevisiae Proteins* ; Signal Recognition Particle*; Mammals/*genetics ; Protein Sorting Signals/*genetics ; Saccharomyces cerevisiae/*genetics; Amino Acid Sequence ; Animals ; Base Sequence ; Blotting, Northern ; Blotting, Southern ; DNA, Fungal/genetics ; Deoxyribonuclease HindIII/metabolism ; Endoplasmic Reticulum/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Molecular Sequence Data ; Nucleic Acid Hybridization ; Sequence Homology, Nucleic Acid ; Translocation, Genetic
    • Abstract:
      A 1.7 kilobase HindIII fragment of Saccharomyces cerevisiae DNA was cloned by cross-hybridization with the Escherichia coli secY gene. The complete nucleotide sequence of the 2.6 kb fragment of the yeast genomic DNA containing the cross-hybridizing HindIII fragment was determined. The sequence showed no apparent similarity with that of the E. coli secY gene with the exception of a completely matched sequence of 21 bp, but it contained a 1,623 nucleotide open reading frame coding for a protein of 541 amino acids with a calculated Mr of 59,600. The N-terminal portion of 303 residues of the predicted sequence was homologous to the cytosolic domain of the alpha-subunit of the signal recognition particle receptor (SR alpha), including consensus sequence elements for a GTP binding site, whereas the C-terminal portion of 238 residues had an unusual methionine-rich domain containing several repetitive sequences. An mRNA of 2.0 kb was detected on Northern blotting analysis. The predicted sequence was 48% identical with the reported sequences of the 54K subunit of the mammalian signal recognition particle (SRP54) (Romisch K. et al. (1989) Nature 340, 478-483; Bernstein, H.D. et al. (1989) Nature 340, 482-486). We designated this gene as SRH1 (SRP54 homologue). Gene disruption experiments showed that the SRH1 gene product is essential for cell growth.
    • Molecular Sequence:
      GENBANK X16908
    • Accession Number:
      0 (DNA, Fungal)
      0 (Fungal Proteins)
      0 (Protein Sorting Signals)
      0 (SRP54 protein, S cerevisiae)
      0 (Saccharomyces cerevisiae Proteins)
      0 (Signal Recognition Particle)
      EC 3.1.21.- (Deoxyribonuclease HindIII)
    • Publication Date:
      Date Created: 19900301 Date Completed: 19900626 Latest Revision: 20190510
    • Publication Date:
      20231215
    • Accession Number:
      10.1093/oxfordjournals.jbchem.a123067
    • Accession Number:
      2187859