Alteration of xylose reductase coenzyme preference to improve ethanol production by Saccharomyces cerevisiae from high xylose concentrations.

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  • Author(s): Xiong M;Xiong M; Chen G; Barford J
  • Source:
    Bioresource technology [Bioresour Technol] 2011 Oct; Vol. 102 (19), pp. 9206-15. Date of Electronic Publication: 2011 Aug 09.
  • Publication Type:
    Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Applied Science Country of Publication: England NLM ID: 9889523 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2976 (Electronic) Linking ISSN: 09608524 NLM ISO Abbreviation: Bioresour Technol Subsets: MEDLINE
    • Publication Information:
      Original Publication: Barking, Essex, England : New York, N.Y. : Elsevier Applied Science ; Elsevier Science Pub. Co., 1991-
    • Subject Terms:
      Biofuels*; Aldehyde Reductase/*metabolism ; Coenzymes/*metabolism ; Ethanol/*metabolism ; Saccharomyces cerevisiae/*metabolism ; Xylose/*metabolism ; Xylulose/*metabolism; Aldehyde Reductase/genetics ; Anaerobiosis ; DNA Primers/genetics ; Fermentation ; Mutagenesis, Site-Directed ; Mutation, Missense/genetics ; Phosphorylation ; Saccharomyces cerevisiae/enzymology ; Species Specificity
    • Abstract:
      A K270R mutation of xylose reductase (XR) was constructed by site-direct mutagenesis. Fermentation results of the F106X and F106KR strains, which carried wild type XR and K270R respectively, were compared using different substrate concentrations (from 55 to 220 g/L). After 72 h, F106X produced less ethanol than xylitol, while F106KR produced ethanol at a constant yield of 0.36 g/g for all xylose concentrations. The xylose consumption rate and ethanol productivity increased with increasing xylose concentrations in F106KR strain. In particular, F106KR produced 77.6g/L ethanol from 220 g/L xylose and converted 100 g/L glucose and 100g/L xylose into 89.0 g/L ethanol in 72h, but the corresponding values of F106X strain are 7.5 and 65.8 g/L. The ethanol yield of F106KR from glucose and xylose was 0.42 g/g, which was 82.3% of the theoretical yield. These results suggest that the F106KR strain is an excellent producer of ethanol from xylose.
      (Copyright © 2011 Elsevier Ltd. All rights reserved.)
    • Accession Number:
      0 (Biofuels)
      0 (Coenzymes)
      0 (DNA Primers)
      3K9958V90M (Ethanol)
      5962-29-8 (Xylulose)
      A1TA934AKO (Xylose)
      EC 1.1.1.21 (Aldehyde Reductase)
    • Publication Date:
      Date Created: 20110812 Date Completed: 20120105 Latest Revision: 20220321
    • Publication Date:
      20231215
    • Accession Number:
      10.1016/j.biortech.2011.06.058
    • Accession Number:
      21831633