Embryological manipulations in zebrafish.

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Sun Y;Sun Y; Wloga D; Dougan ST
  • Source:
    Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2011; Vol. 770, pp. 139-84.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
    • Publication Information:
      Publication: Totowa, NJ : Humana Press
      Original Publication: Clifton, N.J. : Humana Press,
    • Subject Terms:
      Embryo, Nonmammalian/*cytology ; Embryo, Nonmammalian/*metabolism ; Embryology/*methods ; Zebrafish/*embryology; Animals ; Cell Differentiation ; Color ; Embryo, Nonmammalian/transplantation ; Fluorescent Antibody Technique ; Gene Expression Profiling ; Immunohistochemistry ; In Situ Hybridization ; Microinjections ; RNA Probes/biosynthesis ; RNA Probes/metabolism ; RNA, Messenger/biosynthesis
    • Abstract:
      Due to the powerful combination of genetic and embryological techniques, the teleost fish Danio rerio has emerged in the last decade as an important model organism for the study of embryonic development. It is relatively easy to inject material such as mRNA or synthetic oligonucleotides to reduce or increase the expression of a gene product. Changes in gene expression can be analyzed at the level of mRNA, by whole-mount in situ hybridization, or at the level of protein, by immunofluorescence. It is also possible to quantitatively analyze protein levels by Western and immunoprecipitation. Cell behavior can be analyzed in detail by cell transplantation and by fate mapping. Because a large number of mutations have been identified in recent years, these methods can be applied in a variety of contexts to provide a deep understanding of gene function that is often more difficult to achieve in other vertebrate model systems.
    • Accession Number:
      0 (RNA Probes)
      0 (RNA, Messenger)
    • Publication Date:
      Date Created: 20110802 Date Completed: 20111115 Latest Revision: 20110801
    • Publication Date:
      20231215
    • Accession Number:
      10.1007/978-1-61779-210-6_6
    • Accession Number:
      21805264