Isoflurane preconditioning involves upregulation of molecular chaperone genes.

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  • Author(s): McClintick CA;McClintick CA; Theisen CS; Ferns JE; Fibuch EE; Seidler NW
  • Source:
    Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Jul 29; Vol. 411 (2), pp. 387-92. Date of Electronic Publication: 2011 Jun 29.
  • Publication Type:
    Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE
    • Publication Information:
      Publication: <2002- >: San Diego, CA : Elsevier
      Original Publication: New York, Academic Press.
    • Subject Terms:
      Cytoprotection*; Anesthetics, Inhalation/*pharmacology ; Gene Expression/*drug effects ; Isoflurane/*pharmacology ; Molecular Chaperones/*genetics ; Neurons/*drug effects; Apoptosis/drug effects ; Cell Line, Tumor ; Cell Survival/drug effects ; Heat-Shock Proteins/genetics ; Humans ; Neurons/physiology ; Protein Folding/drug effects ; Up-Regulation
    • Abstract:
      Isoflurane preconditioning is a phenomenon in which cells previously exposed to isoflurane exhibit protection against subsequent noxious stimuli. We hypothesize that isoflurane may cause subtle protein misfolding that persists at a sublethal level, stimulating cytoprotective mechanisms. Human neuroblastoma cells (SH-SY5Y) were exposed to isoflurane followed by quantitative analysis of the expression of several families of heat shock genes (84 total transcripts). Our data is consistent with a model of an early and delayed phase of preconditioning. Different patterns of expression of the 84 genes were seen at 1 and 24h post-isoflurane exposure. Expression of 45 of the 84 genes were elevated at 1h (or early phase) and remained upregulated at 24h (or delayed phase). Subsets of the remaining genes were either unchanged (13 genes), early-specific upregulated (17 genes) or delayed-specific upregulated (9 genes). We also demonstrated that isoflurane caused a slight yet detectable misfold of a model protein. These data indicate that brief anesthetic exposure promotes specific patterns of gene expression, leading to preconditioning which would enhance the cell's ability to tolerate a future injury that involves protein misfolding.
      (Copyright © 2011 Elsevier Inc. All rights reserved.)
    • Accession Number:
      0 (Anesthetics, Inhalation)
      0 (Heat-Shock Proteins)
      0 (Molecular Chaperones)
      CYS9AKD70P (Isoflurane)
    • Publication Date:
      Date Created: 20110712 Date Completed: 20111003 Latest Revision: 20131121
    • Publication Date:
      20240829
    • Accession Number:
      10.1016/j.bbrc.2011.06.156
    • Accession Number:
      21741358