Drug quantification in turbid media by fluorescence imaging combined with light-absorption correction using white Monte Carlo simulations.

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  • Additional Information
    • Source:
      Publisher: Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society Country of Publication: United States NLM ID: 9605853 Publication Model: Print Cited Medium: Internet ISSN: 1560-2281 (Electronic) Linking ISSN: 10833668 NLM ISO Abbreviation: J Biomed Opt Subsets: MEDLINE
    • Publication Information:
      Original Publication: Bellingham, WA : Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society, c1996-
    • Subject Terms:
      Monte Carlo Method* ; Signal Processing, Computer-Assisted*; Photosensitizing Agents/*chemistry ; Spectrometry, Fluorescence/*methods; Absorption ; Algorithms ; Animals ; Chromatography, High Pressure Liquid ; Computer Simulation ; Drug Monitoring ; Female ; Fluorescent Dyes/analysis ; Fluorescent Dyes/chemistry ; Fluorescent Dyes/pharmacokinetics ; Mice ; Mice, Nude ; Models, Biological ; Phantoms, Imaging ; Photochemotherapy ; Photosensitizing Agents/analysis ; Photosensitizing Agents/pharmacokinetics ; Rhodamines/analysis ; Rhodamines/chemistry ; Rhodamines/pharmacokinetics ; Tissue Distribution
    • Abstract:
      Accurate quantification of photosensitizers is in many cases a critical issue in photodynamic therapy. As a noninvasive and sensitive tool, fluorescence imaging has attracted particular interest for quantification in pre-clinical research. However, due to the absorption of excitation and emission light by turbid media, such as biological tissue, the detected fluorescence signal does not have a simple and unique dependence on the fluorophore concentration for different tissues, but depends in a complex way on other parameters as well. For this reason, little has been done on drug quantification in vivo by the fluorescence imaging technique. In this paper we present a novel approach to compensate for the light absorption in homogeneous turbid media both for the excitation and emission light, utilizing time-resolved fluorescence white Monte Carlo simulations combined with the Beer-Lambert law. This method shows that the corrected fluorescence intensity is almost proportional to the absolute fluorophore concentration. The results on controllable tissue phantoms and murine tissues are presented and show good correlations between the evaluated fluorescence intensities after the light-absorption correction and absolute fluorophore concentrations. These results suggest that the technique potentially provides the means to quantify the fluorophore concentration from fluorescence images.
    • Accession Number:
      0 (Fluorescent Dyes)
      0 (Photosensitizing Agents)
      0 (Rhodamines)
      037VRW83CF (rhodamine 6G)
    • Publication Date:
      Date Created: 20110705 Date Completed: 20111108 Latest Revision: 20190930
    • Publication Date:
      20221213
    • Accession Number:
      10.1117/1.3585675
    • Accession Number:
      21721803