Endothelin stimulation of cytosolic calcium and gonadotropin secretion in anterior pituitary cells.

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  • Additional Information
    • Source:
      Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print Cited Medium: Print ISSN: 0036-8075 (Print) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE
    • Publication Information:
      Publication: : Washington, DC : American Association for the Advancement of Science
      Original Publication: New York, N.Y. : [s.n.] 1880-
    • Subject Terms:
      Calcium/*metabolism ; Follicle Stimulating Hormone/*metabolism ; Luteinizing Hormone/*metabolism ; Peptides/*pharmacology ; Pituitary Gland, Anterior/*metabolism; Animals ; Cells, Cultured ; Cytosol/metabolism ; Endothelins ; Endothelium, Vascular ; Female ; Gonadotropin-Releasing Hormone/pharmacology ; Kinetics ; Male ; Nifedipine/pharmacology ; Orchiectomy ; Peptides/metabolism ; Pituitary Gland, Anterior/drug effects ; Rats ; Rats, Inbred Strains ; Receptors, Cell Surface/metabolism ; Receptors, Endothelin ; Reference Values
    • Abstract:
      The presence of endothelin, a vasoconstrictor peptide, in the hypothalamus and posterior pituitary suggests that it also regulates neural and other nonvascular target cells. In pituitary gonadotrophs, low doses of endothelin evoked oscillations in the intracellular calcium concentration, and high doses induced a biphasic calcium response. Mobilization of intracellular calcium predominated during the spike phase of the calcium response to endothelin, whereas calcium entry through dihydropyridine-sensitive channels contributed to both the spike and plateau phases of the calcium response. Endothelin was a potent as hypothalamic gonadotropin-releasing hormone (GnRH) in stimulation of gonadotropin release in perifused pituitary cells. Endothelin bound specifically to pituitary cells with a dissociation constant of 70 picomolar, and induced rapid formation of inositol trisphosphate and diacyglycerol. Although intracellular calcium concentration and gonadotropin secretory responses to endothelin were independent to the GnRH receptor, endothelin and GnRH appeared to have a common signal transduction mechanism. These observations suggest that endothelin can act as a neuropeptide to regulate anterior pituitary function.
    • Accession Number:
      0 (Endothelins)
      0 (Peptides)
      0 (Receptors, Cell Surface)
      0 (Receptors, Endothelin)
      33515-09-2 (Gonadotropin-Releasing Hormone)
      9002-67-9 (Luteinizing Hormone)
      9002-68-0 (Follicle Stimulating Hormone)
      I9ZF7L6G2L (Nifedipine)
      SY7Q814VUP (Calcium)
    • Publication Date:
      Date Created: 19900629 Date Completed: 19900806 Latest Revision: 20190618
    • Publication Date:
      20221213
    • Accession Number:
      10.1126/science.2163546
    • Accession Number:
      2163546