Vasoactive intestinal peptide is a hypothalamic prolactin-releasing neuropeptide in the turkey (Meleagris gallopavo).

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  • Author(s): el Halawani ME;el Halawani ME; Silsby JL; Mauro LJ
  • Source:
    General and comparative endocrinology [Gen Comp Endocrinol] 1990 Apr; Vol. 78 (1), pp. 66-73.
  • Publication Type:
    Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • Language:
    English
  • Additional Information
    • Source:
      Publisher: Academic Press Country of Publication: United States NLM ID: 0370735 Publication Model: Print Cited Medium: Print ISSN: 0016-6480 (Print) Linking ISSN: 00166480 NLM ISO Abbreviation: Gen Comp Endocrinol Subsets: MEDLINE
    • Publication Information:
      Publication: New York, NY : Academic Press
      Original Publication: New York.
    • Subject Terms:
      Hypothalamus/*physiology ; Prolactin/*metabolism ; Turkeys/*physiology ; Vasoactive Intestinal Peptide/*physiology; Animals ; Female ; Pituitary Gland, Anterior/drug effects ; Pituitary Gland, Anterior/metabolism ; Receptors, Gastrointestinal Hormone/drug effects ; Receptors, Gastrointestinal Hormone/physiology ; Receptors, Vasoactive Intestinal Peptide ; Vasoactive Intestinal Peptide/pharmacology
    • Abstract:
      The hypothesis that vasoactive intestinal peptide (VIP) functions as a hypothalamic prolactin (PRL)-releasing peptide in the turkey was tested by determining the effects of hypothalamic VIP immunoneutralization and pituitary VIP receptor blockade on hypothalamic extract (HE)-induced PRL secretion from dispersed anterior pituitaries. Incubation of cells with porcine VIP (pVIP; 0.5 or 10 nM) significantly stimulated PRL secretion. This effect was inhibited in a dose-related manner by 1-hr preincubation of pVIP with a VIP antisera (A/S; 1:500-1:50,000). Likewise, HE (0.3 equivalent)-stimulated PRL secretion was inhibited by preincubation with VIP A/S (P less than 0.0001). A 96-98% reduction in PRL secretion was obtained from cells cultured with HE, that was previously incubated with 1/500 dilution of antiserum. Pretreatment of pituitary cells for 15 min with [4Cl-D-Phe6,Leu17] VIP, a VIP receptor antagonist (10(-5) M), significantly depressed the PRL response to 0.5 nM VIP (9.9 +/- 0.5 micrograms/500,000 cells vs 4.9 +/- 0.1 micrograms/500,000 cells; 22.4 +/- 0.9 micrograms/500,000 cells vs 14.7 +/- 0.4 micrograms/500,000 cells) or 0.3 eq HE (8.8 +/- 0.6 micrograms/500,000 cells vs 5.2 +/- 0.2 micrograms/500,000 cells; 15.3 +/- 0.3 micrograms/500,000 cells vs 8.2 +/- 0.2 micrograms/500,000 cells). These results suggest that hypothalamic stimulation of PRL secretion appears to be mediated by receptors specific for VIP and that VIP is an endogenous hypothalamic PRL-releasing peptide in the turkey.
    • Accession Number:
      0 (Receptors, Gastrointestinal Hormone)
      0 (Receptors, Vasoactive Intestinal Peptide)
      102805-45-8 (vasoactive intestinal peptide, 4-chloro-Phe(6)-Leu(17)-)
      37221-79-7 (Vasoactive Intestinal Peptide)
      9002-62-4 (Prolactin)
    • Publication Date:
      Date Created: 19900401 Date Completed: 19900606 Latest Revision: 20190814
    • Publication Date:
      20221208
    • Accession Number:
      10.1016/0016-6480(90)90048-q
    • Accession Number:
      2158920