Diverse Helotiales associated with the roots of three species of Arctic Ericaceae provide no evidence for host specificity.

Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE

Publication: Oxford : Wiley on behalf of New Phytologist Trust
Original Publication: London, New York [etc.] Academic Press.

Ascomycota/*classification ; Basidiomycota/*classification ; Ericaceae/*microbiology ; Mycorrhizae/*classification; Alaska ; Arctic Regions ; Ascomycota/genetics ; Ascomycota/isolation & purification ; Base Sequence ; Basidiomycota/genetics ; Basidiomycota/isolation & purification ; DNA, Fungal/chemistry ; DNA, Fungal/genetics ; Ecosystem ; Host Specificity ; Molecular Sequence Data ; Mycological Typing Techniques ; Mycorrhizae/genetics ; Mycorrhizae/isolation & purification ; Phylogeny ; Plant Roots/genetics ; Plant Roots/microbiology ; Sequence Analysis, DNA ; Symbiosis

Ericoid mycorrhizal fungi differ in their abilities to use nitrogen sources and may be integral to maintaining fungal and plant diversity in ecosystems in which Ericaceae occur. In this study, we tested whether the fungal communities differ among three species of co-occurring Ericaceae. Fungi colonizing Cassiope tetragona, Empetrum nigrum and Vaccinium vitis-idaea roots in the Arctic tundra were characterized via culture-dependent and culture-independent techniques. The cultured fungi were tested for their ability to colonize Vaccinium uliginosum in laboratory-based assays. The pure-cultured Helotiales were grouped into eight clades and dominated by the Phialocephala-Acephala complex. Representatives of these clades, plus an unknown basidiomycete with affinity to the genus Irpex (Polyporales), colonized V. uliginosum intracellularly. The Helotiales detected by direct PCR, cloning and sequencing were assigned to 14 clades and dominated by members of the Rhizoscyphus ericae complex. Ordination analyses indicated that culture-dependent and culture-independent assays provided distinct views of root fungal communities, but no evidence for host specificity. These data suggest that ericaceous roots host diverse fungal communities dominated by the Helotiales. However, these fungal communities are unlikely to be controlled by fungal host preferences. The mechanisms maintaining high diversity in root-symbiotic communities remain to be elucidated.
(© 2011 The Authors. New Phytologist © 2011 New Phytologist Trust.)

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0 (DNA, Fungal)

Date Created: 20110406 Date Completed: 20130328 Latest Revision: 20210423

20231215

10.1111/j.1469-8137.2011.03703.x

21463329