[Analysis on the metabolites of mesaconitine in the rat urine by liquid chromatography and electrospray ionization mass spectrometry].

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
Read More Add to Saved list
  • Author(s): Chen PP;Chen PP; Zhao N; Xu XL; Ruan YP; Wei YH; Li FZ
  • Source:
    Yao xue xue bao = Acta pharmaceutica Sinica [Yao Xue Xue Bao] 2010 Aug; Vol. 45 (8), pp. 1043-7.
  • Publication Type:
    English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • Language:
    Chinese
  • Additional Information
    • Source:
      Publisher: Zhongguo yao xue hui Country of Publication: China NLM ID: 21710340R Publication Model: Print Cited Medium: Print ISSN: 0513-4870 (Print) Linking ISSN: 05134870 NLM ISO Abbreviation: Yao Xue Xue Bao Subsets: MEDLINE
    • Publication Information:
      Original Publication: Beijing : Zhongguo yao xue hui
    • Subject Terms:
      Chromatography, High Pressure Liquid* ; Spectrometry, Mass, Electrospray Ionization*; Aconitine/*analogs & derivatives ; Aconitum/*chemistry; Aconitine/isolation & purification ; Aconitine/metabolism ; Aconitine/urine ; Animals ; Female ; Male ; Molecular Structure ; Plants, Medicinal/chemistry ; Rats ; Rats, Sprague-Dawley ; Solid Phase Extraction
    • Abstract:
      The mesaconitine and its major metabolites in the rat urine were identified by liquid chromatography and electrospray ionization tandem mass spectrometry. The rat urine was collected for consecutive 24 hours from the rat following intragastric infusion of mesaconitine, subsequently which were enriched and purified using solid phase extraction. The metabolites of mesaconitine in the rat urine were analyzed by the liquid chromatography and electrospray ionization tandem mass spectrometry. It is shown that the parent drug mesaconitine and its metabolites were found in the rat urine, such as hypo-mesaconitine glucuronic acid conjugate, 10-hydroxy-mesaconitine, 1-O-demethyl mesaconitine, deoxy-mesaconitine and hypo-mesaconitine. Among the five of metabolites, the hypo-mesaconitine glucuronic acid conjugate (m/z 766) was first discovered as the aconitine in rats phase II metabolites, which revealed a new way of mesaconitine metabolism in rats.
    • Accession Number:
      621OT356HL (mesaconitine)
      X8YN71D5WC (Aconitine)
    • Publication Date:
      Date Created: 20110301 Date Completed: 20120816 Latest Revision: 20151119
    • Publication Date:
      20240829
    • Accession Number:
      21351593